Raf-1 kinase inhibitory protein (RKIP) acts as a tumor cell metastasis suppressor and prognostic indicator for survival in various cancers. Its use is predicted to improve therapy for various malignancies, including colorectal cancer (CRC). RKIP, frequently denoted as phosphatidylethanolamine-binding protein 1, is expressed in all normal mammalian tissues. RKIP functions as an inhibitor of the Raf-1, PI-3K, and MAP kinase (MAPK) pathways. In this study, we found that resveratrol induced the expression of RKIP at protein levels. To elucidate the structural basis of the interaction between resveratrol and RKIP, we performed computational studies that explore the binding affinity and ligand efficacy of resveratrol against RKIP. This study reveals the prognostic significance of RKIP metastasis suppressor activity against CRC and its structural arrangements during drug–target interactions.

Raf-1激酶抑制蛋白（RKIP）充当肿瘤细胞转移抑制物和各种癌症存活率的预后指标。预计将其用于改善各种恶性肿瘤的治疗，包括结直肠癌（CRC）。RKIP，通常表示为磷脂酰乙醇胺结合蛋白1，在所有正常的哺乳动物组织中都有表达。RKIP充当Raf-1，PI-3K和MAP激酶（MAPK）途径的抑制剂。在这项研究中，我们发现白藜芦醇可以在蛋白质水平上诱导RKIP的表达。为了阐明白藜芦醇与RKIP之间相互作用的结构基础，我们进行了计算研究，探索白藜芦醇对RKIP的结合亲和力和配体功效。