Gossypol, a toxic polyphenol extracted from cotton seeds, is hazardous to human and animal health. Taurine is considered as an essential or semi-essential amino acid and has diverse cytoprotective effects. This study was aimed to investigate the protective effect and molecular mechanism of taurine against apoptosis of C2C12 mouse myoblasts induced by gossypol. C2C12 mouse myoblasts were exposed to gossypol (0, 1 nM, 10 nM, 100 nM, 1 μM, and 10 μM). Cell numbers were rapidly decreased with increasing concentrations of gossypol. Gossypol significantly induced apoptosis, decreased Bcl2 expression, and increased the protein levels of Bax and the cleaved caspase 3. Taurine (0.24 mM) treatment largely rescued the cell number decreased by gossypol, attenuated gossypol-induced cell apoptosis. GPR87 knockdown abolished the inhibition by taurine of cell apoptosis. Furthermore, GPR87 overexpression attenuated cell apoptosis induced by gossypol. Both taurine treatment and GPR87 overexpression stimulated AKT phosphorylation but inhibited AMPK phosphorylation, whereas gossypol had the opposite effects. Taurine treatment promoted GPR87 expression and subcellular localization and partially rescued the inhibition of gossypol on this expression. In summary, these data reveal that taurine attenuates gossypol-induced apoptosis of C2C12 mouse myoblasts via the GPR87-AMPK/AKT signaling.

棉酚是一种从棉籽中提取的有毒多酚，对人类和动物健康有害。牛磺酸被认为是必需氨基酸或半必需氨基酸，具有多种细胞保护作用。本研究旨在探讨牛磺酸对棉酚诱导的C2C12小鼠成肌细胞凋亡的保护作用及其分子机制。将C2C12小鼠成肌细胞暴露于棉酚（0、1 nM，10 nM，100 nM，1μM和10μM）。随着棉酚浓度的增加，细胞数量迅速减少。棉酚显着诱导了细胞凋亡，降低了Bcl2表达，并增加了Bax和裂解的半胱天冬酶3的蛋白水平。牛磺酸（0.24 mM）处理在很大程度上拯救了棉酚减少的细胞数量，减弱了棉酚诱导的细胞凋亡。GPR87敲低取消了牛磺酸对细胞凋亡的抑制作用。此外，GPR87过表达减弱了棉酚诱导的细胞凋亡。牛磺酸处理和GPR87过表达均刺激AKT磷酸化，但抑制AMPK磷酸化，而棉酚则相反。牛磺酸处理促进了GPR87表达和亚细胞定位，并部分缓解了棉酚对该表达的抑制作用。总之，这些数据表明牛磺酸通过GPR87-AMPK / AKT信号传导减弱了棉酚诱导的C2C12小鼠成肌细胞凋亡。牛磺酸处理促进了GPR87表达和亚细胞定位，并部分缓解了棉酚对该表达的抑制作用。总之，这些数据表明牛磺酸通过GPR87-AMPK / AKT信号传导减弱了棉酚诱导的C2C12小鼠成肌细胞凋亡。牛磺酸处理促进了GPR87表达和亚细胞定位，并部分缓解了棉酚对该表达的抑制作用。总之，这些数据表明牛磺酸通过GPR87-AMPK / AKT信号传导减弱了棉酚诱导的C2C12小鼠成肌细胞凋亡。