The study reports protective role of potential probiotic cultures against infection by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model system. Among the fifteen indigenous potential probiotics, the cell free supernatant of Lactobacillus gastricus BTM7 possessed highest antimicrobial action and biofilm inhibition against C. sakazakii. The competitive exclusion assays revealed that preconditioning with probiotics resulted in increased mean life span of the nematode to 12–13 days as compared to 5–6 days when the pathogen was administered alone. Enhanced expression of the marker genes (pmk-1, daf-16 and skn-1) was observed during the administration of probiotic cultures. The highest expression of pmk-1 (2.5 folds) was observed with administration of L. gastricus BTM7. The principal component analysis on selected variables revealed that L. gastricus BTM7 has the potential to limit the infection of C. sakazakii in C. elegans and enhance the expression of key genes involved in extending life span of the worm.

该研究报告对潜在感染益生菌培养生物膜形成的保护作用坂崎肠杆菌在秀丽隐杆线虫模型系统。在15种潜在的本土益生菌中，胃乳酸杆菌BTM7的无细胞上清液对阪崎肠杆菌具有最高的抗菌作用和生物膜抑制作用。竞争性排斥试验表明，用益生菌进行预处理可使线虫的平均寿命延长至12-13天，而单独施用病原体则为5-6天。在施用益生菌培养物期间观察到标记基因（pmk-1，daf-16和skn-1）的表达增强。胃泌乳芽孢杆菌BTM7给药时，观察到pmk-1的最高表达（2.5倍）。对选定变量的主成分分析表明，胃L.胃BTM7有潜力限制秀丽隐杆线虫感染阪崎肠杆菌，并增强与延长蠕虫寿命有关的关键基因的表达。