Abstract

To test the reliability of Restriction Fragment Length Polymorphism data for ATPase6–NADH4L region of mitochondrial DNA (2162 bp), which unambiguously support the allopatric origin of the small-mouth charr Salvelinus elgyticus and “Boganida” charr S. “boganidae” in Lake Elgygytgyn, this region was sequenced in some members of the S. alpinus–S. malma complex. It is shown that RFLP data on the number of restriction sites and the size of restriction fragments for the 2162 bp region are poorly consistent with data on nucleotide sequences for the majority of forms and species of charr. Based on the identified errors, data on the MvaI restriction sites for ATPase6–NADH4L region of mitochondrial DNA cannot be considered as unambiguous evidence in favor of the allopatric origin of the two charr species in Lake Elgygytgyn. Nevertheless, the nucleotide data for this fragment suggest with a high probability that the small-mouth and “Boganida” charrs of Lake Elgygytgyn have an allopatric origin.

中文翻译：

---

关于Elgygytgyn湖中两种Chars（Salvelinus，Salmonidae）的起源：线粒体DNA ATPase6 – NADH4L区的数据

摘要

测试线粒体DNA ATPase6 – NADH4L区域（2162 bp）的限制性片段长度多态性数据的可靠性，这些数据明确支持小口目沙尔Salvelinus elgyticus和“ Boganida ” charr S.“ boganidae”的异源起源，该区域在高山链球菌的某些成员中进行了测序。马尔玛情结。结果表明，关于2162 bp区域的限制性酶切位点数目和限制性片段大小的RFLP数据与大多数charr形式和种类的核苷酸序列数据不一致。基于已识别的错误，ATPase6的MvaI限制性酶切位点上的数据–不能认为线粒体DNA的NADH4L区是支持Elgygytgyn湖中两种charr物种的异源来源的明确证据。然而，该片段的核苷酸数据极有可能暗示了埃尔吉特金湖的小嘴和“ Boganida”炭黑具有异源性。