Nitrogen (N) is an essential nutrient for plant growth. A modified up-down two-compartment system, with the addition of 15N-labelled wheat straw in the hyphal compartment (HC), was set up to investigate the effects of vermicompost and arbuscular mycorrhizal (AM) fungi on improving 15N mineralisation from wheat straw and uptake by lettuce. Lettuce yield, the 15N contents of shoots and roots, the concentration of inorganic 15N in soil, the percentage of 15N transfer were measured, as well as soil organic matter content, soil aggregate-size distribution, hyphal length density and the soil bacterial community in the HC. Vermicompost can improve soil aggregation by replenishing organic matter, which can subsequently regulate the soil bacterial community and increase the relative abundance of N-cycling bacteria in the hyphosphere. Vermicompost enhanced 15N mineralisation from wheat straw by regulating the physical structure of the soil and the bacterial community but did not help transferring 15N to the plant alone. AM fungi hyphae could transfer 15N from the HC to the plant, which significantly increased shoot and root 15N uptake. Ultimately, the interaction between vermicompost and AM fungi increased the yield of lettuce by enhancing the uptake of N that was mineralised from crop residues. Overall, the interaction between vermicompost and AM fungi may help lettuce to speed up 15N acqusition from crop residues via mineralization induced by vermicompost amendment and the transfer from soil to plant via AM fungi.

中文翻译：

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Vermicompost 辅助丛枝菌根真菌将 15N 从作物残留物转移到生菜

氮（N）是植物生长必不可少的营养元素。建立改良的上下两室系统，在菌丝室 (HC) 中添加 15N 标记的小麦秸秆，研究蚯蚓粪和丛枝菌根 (AM) 真菌对提高小麦秸秆 15N 矿化的影响并被生菜吸收。测定生菜产量、地上部和根部15N含量、土壤中无机15N浓度、15N转移百分比，以及土壤有机质含量、土壤团聚体粒径分布、菌丝长度密度和土壤细菌群落。 HC。蚯蚓堆肥可以通过补充有机质来改善土壤团聚，进而调节土壤细菌群落，增加下层氮循环细菌的相对丰度。蚯蚓粪通过调节土壤和细菌群落的物理结构增强了小麦秸秆的 15N 矿化，但无助于将 15N 单独转移到植物中。AM 真菌菌丝可以将 15N 从 HC 转移到植物中，这显着增加了地上部和根部对 15N 的吸收。最终，蚯蚓堆肥和 AM 真菌之间的相互作用通过增强从作物残留物中矿化的 N 的吸收来增加生菜的产量。总体而言，蚯蚓粪和 AM 真菌之间的相互作用可能有助于生菜通过蚯蚓粪改良剂诱导的矿化和通过 AM 真菌从土壤转移到植物中，加速从作物残留物中获取 15N。AM 真菌菌丝可以将 15N 从 HC 转移到植物中，这显着增加了地上部和根部 15N 的吸收。最终，蚯蚓堆肥和 AM 真菌之间的相互作用通过增强从作物残留物中矿化的 N 的吸收来增加生菜的产量。总体而言，蚯蚓粪和 AM 真菌之间的相互作用可能有助于生菜通过蚯蚓粪改良剂诱导的矿化和通过 AM 真菌从土壤转移到植物中，加速从作物残留物中获取 15N。AM 真菌菌丝可以将 15N 从 HC 转移到植物中，这显着增加了地上部和根部 15N 的吸收。最终，蚯蚓堆肥和 AM 真菌之间的相互作用通过增强从作物残留物中矿化的 N 的吸收来增加生菜的产量。总体而言，蚯蚓粪和 AM 真菌之间的相互作用可能有助于生菜通过蚯蚓粪改良剂诱导的矿化和通过 AM 真菌从土壤转移到植物中，加速从作物残留物中获取 15N。