Monitoring of bisphenols (BPs) in feed is a necessary process for the security of food of animal origin. In this study, a method was established to simultaneously detect bisphenol A (BPA), bisphenol B (BPB), bisphenol C (BPC), bisphenol E (BPE), bisphenol F (BPF), bisphenol AP (BPAP), bisphenol Z (BPZ), bisphenol P (BPP) and bisphenol PH (BPPH) in feed. A simple and rapid pretreatment procedure was developed using ultrasound-assisted extraction (UAE) and dispersive solid-phase extraction (DSPE), and then the prepared sample was analysed by high-performance liquid chromatographic (HPLC) with fluorescence detector (FLD). Finally, the operating conditions of UAE, DSPE, and HPLC were all optimised to increase the sample throughput and sensitivity. Under the optimized conditions, good linearities were obtained for nine BPs with the correlation coefficients (R2) above 0.995; the recoveries of nine BPs in spiked feed samples were in the range of 82.56–103.87% and relative standard deviations (RSDs) were in the range of 2.4–13.40%; the limits of detections (LODs) and quantitations (LOQs) were in the range of 1.0–5.0 and 3.3–16.6 μg kg − 1, respectively. The results clearly demonstrated the developed method provided the reliable screening and quantification of nine BPs in feed and possessed the advantages including low cost, quick, and simple.

中文翻译：

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超声辅助萃取和分散固相萃取-高效液相色谱-荧光检测器测定饲料中双酚类化合物的多残留

监测饲料中的双酚 (BPs) 是动物源性食品安全的必要过程。本研究建立了一种同时检测双酚 A (BPA)、双酚 B (BPB)、双酚 C (BPC)、双酚 E (BPE)、双酚 F (BPF)、双酚 AP (BPAP)、双酚 Z ( BPZ)、双酚 P (BPP) 和双酚 PH (BPPH)。使用超声辅助萃取 (UAE) 和分散固相萃取 (DSPE) 开发了一种简单快速的前处理程序，然后通过配备荧光检测器 (FLD) 的高效液相色谱 (HPLC) 分析制备的样品。最后，UAE、DSPE 和 HPLC 的操作条件都经过优化，以提高样品通量和灵敏度。在优化条件下，9 个 BP 获得了良好的线性，相关系数 (R2) 高于 0.995；加标饲料样品中 9 种 BP 的回收率范围为 82.56-103.87%，相对标准偏差 (RSD) 范围为 2.4-13.40%；检测限 (LOD) 和定量 (LOQ) 分别在 1.0–5.0 和 3.3–16.6 μg kg − 1 的范围内。结果清楚地表明，所开发的方法可以可靠地筛选和定量饲料中的 9 种 BP，并具有成本低、快速、简单等优点。分别。结果清楚地表明，所开发的方法可以可靠地筛选和定量饲料中的 9 种 BP，并具有成本低、快速、简单等优点。分别。结果清楚地表明，所开发的方法可以可靠地筛选和定量饲料中的 9 种 BP，并具有成本低、快速、简单等优点。