A Schiff base of 4E,10E-4-(2-(4-nitrophenyl)-N-((1H-indol-3-yl)methylene) benzenamine was synthesized and characterized by ¹H&¹³C-NMR, FT-IR, and ESI–MS spectroscopy. UV–visible and fluorescence studies were explored for chemosensor ICPA to detect Ni²⁺, Fe³⁺, and Mg²⁺ by fluorescence chemosensor “turn-on” method in 10 mM HEPES buffer in EtOH/H₂O (1:4, v/v) medium. Chemosensor ICPA binds with the selective heavy metal ions and could be proved in very low detection limits. Job’s plot demonstrates 1:1 stoichiometric complexes of ICPA + Ni²⁺, ICPA + Fe³⁺, and ICPA + Fe³⁺ with the calculated mole fraction of 0.5 μM. Binding constant values calculated using the Benesi–Hildebrand method are found to be 3.45 × 10⁸ M⁻², 5.63 × 10⁴ M⁻², and 8.63 × 10³ M⁻², respectively. Competitive metal ions tests were also carried out with transition metal ions. Furthermore, HeLa cell activity at different concentrations added with ligand to perform inhibition capability of the probe was also carried out.

合成了4 E，10 E -4-（2-（4-硝基苯基）-N -（（1H-吲哚-3-基）亚甲基）苯甲胺的席夫碱，并通过¹ H＆¹³ C-NMR，FT-进行了表征红外光谱和ESI-MS光谱学，探索了化学传感器ICPA的紫外-可见和荧光研究，通过荧光化学传感器“开启”方法在EtOH / 10 mM HEPES缓冲液中检测Ni 2 ⁺，Fe ³⁺和Mg ²⁺。 H ₂ O（1：4，v / v）介质化学传感器ICPA与选择性重金属离子结合，可以在非常低的检出限中得到证明。Job的图显示了ICPA +  Ni 2 ⁺，ICPA +  Fe ³⁺和ICPA +  Fe ^3+的1：1化学计量配合物，计算出的摩尔分数为0.5μM。结合使用Benesi-希尔德布兰德方法计算的常数值被发现是3.45×10 ⁸ 中号^-2，5.63×10 ⁴ 中号^-2和8.63×10 ³ 中号^-2， 分别。还使用过渡金属离子进行了竞争性金属离子测试。此外，还进行了添加有配体的不同浓度的HeLa细胞活性以执行探针的抑制能力。