Abstract Estrone, 17β-estradiol, 17α-ethynylestradiol, alkylphenols and alkylphenol ethoxylates, are endocrine disruptor compounds. In Portugal, the report of male fish feminization in Minho, Ave and Mondego River estuaries made pertinent their evaluation in surface water samples. Data, got by specific enzyme-linked immunosorbent assays, showed that estrogens concentrations were lower in Minho than in Ave or Mondego estuaries: [Estrone] > [17β-estradiol] > [17α-ethynylestradiol] (p < 0.05). Converting estrogen concentrations in 17α-ethynylestradiol equivalents, the contribution of estrogens was 1.3 ng/L, 3.5 ng/L, and 2.4 ng/L, respectively, for Minho, Ave, and Mondego estuaries stressing out a high risk for local aquatic species. The concentrations of alkylphenols and alkylphenol ethoxylates were lower at both Minho and Mondego estuaries ≈600 ng/L and 2700 ng/L, respectively than at the Ave, ≈1070 ng/L and 4855 ng/L (p < 0.05). In conclusion, this study reports the first systematic data about the presence of the targeted endocrine disruptor compounds in the Minho River estuary and, in parallel, provides new information about their concentrations in two other habitats previously monitored. This study also shows that in Ave and Mondego estuaries the concentrations of industrial estrogens were still excessive, whereas the load of the pure estrogens has decreased significantly facing previous surveys.

中文翻译：

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伊比利亚半岛西北部（葡萄牙）三个欧洲河口中雌激素内分泌干扰物的存在

摘要 雌酮、17β-雌二醇、17α-乙炔雌二醇、烷基酚和烷基酚乙氧基化物是内分泌干扰物。在葡萄牙，Minho、Ave 和 Mondego River 河口雄鱼雌性化的报告对地表水样本的评估做出了相关的评估。通过特定酶联免疫吸附测定获得的数据表明，Minho 的雌激素浓度低于 Ave 或 Mondego 河口：[雌酮] > [17β-雌二醇] > [17α-乙炔雌二醇] (p < 0.05)。将雌激素浓度转换为 17α-乙炔雌二醇当量，雌激素的贡献分别为 1.3 ng/L、3.5 ng/L 和 2.4 ng/L，对于 Minho、Ave 和 Mondego 河口，强调当地水生物种的高风险。Minho 和 Mondego 河口的烷基酚和烷基酚乙氧基化物的浓度分别 ≈600 ng/L 和 2700 ng/L，比 Ave 的 ≈1070 ng/L 和 4855 ng/L (p < 0.05) 低。总之，这项研究报告了有关 Minho 河口中存在的目标内分泌干扰物化合物的第一个系统数据，同时提供了有关它们在先前监测的其他两个栖息地中的浓度的新信息。这项研究还表明，在 Ave 和 Mondego 河口，工业雌激素的浓度仍然过高，而与之前的调查相比，纯雌激素的负荷已显着下降。这项研究报告了有关 Minho 河口中存在的目标内分泌干扰物化合物的第一个系统数据，同时提供了有关它们在先前监测的其他两个栖息地中的浓度的新信息。这项研究还表明，在 Ave 和 Mondego 河口，工业雌激素的浓度仍然过高，而与之前的调查相比，纯雌激素的负荷已显着下降。这项研究报告了有关 Minho 河口中存在的目标内分泌干扰物化合物的第一个系统数据，同时提供了有关它们在先前监测的其他两个栖息地中的浓度的新信息。这项研究还表明，在 Ave 和 Mondego 河口，工业雌激素的浓度仍然过高，而与之前的调查相比，纯雌激素的负荷已显着下降。