Abstract Glucomannan is a highly branched polysaccharide with glycosidic linkages, constituted of mannoses and glucoses. In recent years, its usefulness due to its immunological, antioxidant and antimutagenic activity has been recognized. The aim of the study was to determine the antigenotoxic ability of glucomannan extracted from Candida utilis orally administered (100–700 mg/kg) to mice, which subsequently received 1 mg/kg aflatoxin B1. Hepatocytes obtained from these animals 4–16 h post administration were examined by means of the comet assay. The antigenotoxic effect was found to be higher than that observed in previous studies with α-mannan and β-D-glucan isolated from Saccharomyces cerevisiae., In order to explore the possibility of formation of a supramolecular complex between glucomannan and aflatoxin B1, both compounds were co-crystallized, their melting points determined, and the complex analyzed through ultraviolet spectroscopy. The spectroscopy data suggest that the protective effect of glucomannan is related to the formation of a supramolecular complex between the two compounds.

中文翻译：

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超分子复合物的形成，葡甘露聚糖对黄曲霉毒素 B1 的一种可能的抗原毒性机制

摘要 葡甘露聚糖是一种具有糖苷键的高度支化的多糖，由甘露糖和葡萄糖构成。近年来，由于其免疫、抗氧化和抗诱变活性，其有用性已得到认可。该研究的目的是确定从念珠菌中提取的葡甘露聚糖对小鼠口服（100-700 毫克/千克），随后接受 1 毫克/千克黄曲霉毒素 B1 的抗原毒性能力。通过彗星试验检查从这些动物给药后 4-16 小时获得的肝细胞。发现其反基因毒性作用高于先前研究中从酿酒酵母中分离的 α-甘露聚糖和 β-D-葡聚糖。为了探索葡甘露聚糖和黄曲霉毒素 B1 之间形成超分子复合物的可能性，将两种化合物共结晶，测定它们的熔点，并通过紫外光谱分析复合物。光谱数据表明，葡甘露聚糖的保护作用与两种化合物之间超分子复合物的形成有关。