Abstract Monoclonal antibodies against the recombinant YB-1 protein were obtained and characterized. These antibodies are capable of specific detection of YB-1 protein in the enzyme-linked immunosorbent assay, Western blot analysis, and immunocytochemistry. Using phage display technology, a peptide interacting with the YB-1 protein was found. Based on monoclonal antibodies МА YB-1 -2 and a peptide as part of the bacteriophage structural protein, a test system was developed for the quantitative determination of YB-1 protein in blood serum. The detection limit of the developed test system is 0.3 ng/mL.

中文翻译：

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单克隆抗体及噬菌体展示技术在YB-1蛋白分析中的应用

摘要 获得并表征了抗重组YB-1蛋白的单克隆抗体。这些抗体能够在酶联免疫吸附测定、蛋白质印迹分析和免疫细胞化学中特异性检测 YB-1 蛋白。使用噬菌体展示技术，发现了与 YB-1 蛋白相互作用的肽。基于单克隆抗体 МА YB-1 -2 和作为噬菌体结构蛋白的一部分的肽，开发了用于定量测定血清中 YB-1 蛋白的测试系统。开发的测试系统的检测限为 0.3 ng/mL。