The paper reports the structural study of the lipopolysaccharide from the type strain of the soil nitrogen-fixing bacterium Azospirillum rugosum isolated from oil-polluted soils. Based on the chemical analysis and 1D- and 2D 1H and 13С NMR spectroscopy data it has been demonstrated that the O-specific polysaccharide consists of the two types of repeating units identical to those previously described for the O-specific polysaccharide of the Azospirillum brasilense strain Jm125A2. Structural analysis of the lipid A using gas-liquid chromatography (GLC) and MALDI mass spectrometry revealed the presence of penta-, tetra-, and triacylated species. The primary fatty acids in the lipid A are the N-linked 16:0(3-OH) and О-linked 14:0(3-OH). Microheterogeneity within each species arises from the presence of different secondary fatty acids (16:0, 18:1, or 19:0) acylating the 16:0(3-OH) acid of the distal GlcN ring.

中文翻译：

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典型菌株 Azospirillum rugosum DSM-19657 的 O 特异性多糖和脂质 A 的结构

本文报道了从油污染土壤中分离出的土壤固氮菌Azospirillum rugosum模式菌株的脂多糖结构研究。基于化学分析和 1D 和 2D 1H 和 13С NMR 光谱数据，已经证明 O 特异性多糖由两种类型的重复单元组成，这些重复单元与先前描述的巴西固氮螺菌菌株的 O 特异性多糖相同Jm125A2。使用气液色谱法 (GLC) 和 MALDI 质谱法对脂质 A 进行结构分析，表明存在五酰化、四酰化和三酰化物质。脂质 A 中的主要脂肪酸是 N-连接的 16:0(3-OH) 和 О-连接的 14:0(3-OH)。每个物种内的微异质性源于不同二级脂肪酸（16:0、18:1、