The aim of this study was to develop alginate and zein films containing natamycin, a natural antifungal agent, in order to limit/prevent the mould growth on the surface of kashar cheeses. The films were prepared by casting, and characterized in terms of antimicrobial and mechanical properties (tensile strength, elongation‐at‐break, and elastic modulus), and their morphology was examined by scanning electron microscopy (SEM). Mechanical properties of the zein films were found to be weaker than the alginate films. SEM analysis indicated that alginate films have a more regular structure than zein films, and a more homogenous distribution was observed at lower concentrations of natamycin. The antifungal activities of both films increased as the natamycin concentration (100, 200, 500, 1000, 2000, and 4000 ppm) increased; however, alginate films exhibited relatively high antifungal activity. The effects of films on the shelf life of kashar cheeses inoculated with Aspergillus niger and Penicillium camemberti were investigated during their storage under refrigerator conditions for 45 days. At high‐natamycin concentrations, zein films showed higher antifungal activity against both fungi at the end of the storage period.

中文翻译：

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评估藻酸盐和玉米蛋白膜作为游霉素的载体以延长卡萨尔干酪的货架期

这项研究的目的是开发含有纳他霉素（一种天然抗真菌剂）的藻酸盐和玉米醇溶蛋白薄膜，以限制/防止卡萨尔干酪表面的霉菌生长。这些薄膜是通过流延制备的，并根据抗菌和机械性能（拉伸强度，断裂伸长率和弹性模量）进行表征，并通过扫描电子显微镜（SEM）对其形态进行了检查。发现玉米醇溶蛋白膜的机械性能比藻酸盐膜弱。SEM分析表明，藻酸盐膜比玉米蛋白膜具有更规则的结构，并且在纳他霉素浓度较低时观察到更均匀的分布。随着纳他霉素浓度（100、200、500、1000、2000和4000 ppm）的增加，两层膜的抗真菌活性均增加。然而，海藻酸盐膜表现出较高的抗真菌活性。薄膜对接种NaCl的卡萨尔奶酪货架期的影响。研究了黑曲霉和卡门培尔青霉在冰箱条件下存放45天的过程。在纳他霉素浓度较高时，玉米醇溶蛋白膜在储存期结束时对两种真菌均显示较高的抗真菌活性。