Abstract

The development and optimization of remote drug delivery systems is a flourishing branch of pharmacy. There is a number of requirements for such systems, among which are biocompatibility and, in some cases, the efficiency of intracellular delivery. The cellular survival after the carrier entrapment is an indirect evidence of biocompatibility of the applied system. The internalization process, as well as main characteristics of carriers themselves can be investigated by imaging flow cytometry and confocal laser scanning microscopy. However, the protocols of carriers’ characterization and the assessment of their uptake efficiency are needed to be improved. In this work, we optimized the evaluation technic for particles’ internalization process by imaging flow cytometry. The confocal laser scanning microscopy was applied as a control method of the particle uptake investigation. The comparative research showed a good correlation between the obtained data. Thus, the high throughput capability of imaging flow cytometry together with the optimized technic of the determination of particle localization inside the cell will further allow us to estimate faster the internalization efficiency of the object under investigation.

中文翻译：

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成像流式细胞仪和共聚焦激光扫描显微镜研究抗真菌载体的细胞摄取

摘要

远程药物输送系统的开发和优化是药学蓬勃发展的一个分支。对于这样的系统有许多要求，其中包括生物相容性以及在某些情况下细胞内递送的效率。载体截留后的细胞存活是所应用系统生物相容性的间接证据。可以通过成像流式细胞仪和共聚焦激光扫描显微镜研究载体的内在化过程以及载体本身的主要特性。然而，运营商的表征协议及其吸收效率的评估有待改进。在这项工作中，我们通过成像流式细胞仪优化了颗粒内在化过程的评估技术。共聚焦激光扫描显微镜被用作颗粒吸收研究的控制方法。比较研究表明所获得的数据之间具有良好的相关性。因此，成像流式细胞仪的高通量能力以及确定细胞内颗粒定位的优化技术将进一步使我们能够更快地估算所研究对象的内在化效率。