Abstract

Using fluorescence methods for visualization of human tumor cell apoptosis and necrosis in vitro, the antitumor effects of flavonoid-containing Gratiola officinalis L. and green tea extracts at ultralow concentrations on A498 human kidney carcinoma cells after 24 and 48 h were compared. Fluorescent dyes (propidium iodide and acridine orange) were used in the “living and dead” test, which made it possible to identify the total number of cells that died by necrosis and apoptosis, as well as cells in which apoptosis has started and apoptotic bodies or pycnosis of the nucleus were detected. Gratiola officinalis L. extract had the most pronounced cytostatic activity 48 h after exposure; the number of cells per field of view decreased by a factor of 1.5 compared with the control. Green tea extract at a concentration of 0.0288 mg/mL had the most pronounced cytotoxic activity; its manifestation was growing 48 h after exposure. Gratiola officinalis L. extract exhibited cytotoxic activity at lower concentrations (0.0036 mg/mL).

中文翻译：

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用荧光成像技术鉴定人参提取物和绿茶类黄酮作用下人肾癌A498细胞的死亡途径

摘要

使用荧光方法可视化体外人肿瘤细胞的凋亡和坏死，比较了含有类黄酮的Gratiola officinalis L.和绿茶提取物以超低浓度对A498人肾癌细胞在24和48小时后的抗肿瘤作用。荧光染料（碘化丙啶和a啶橙）用于“活死”试验，这使得鉴定死于坏死和凋亡的细胞总数以及已经开始凋亡和凋亡小体的细胞成为可能。或检测到核脓肿。香菇暴露后48小时，L。提取物具有最明显的细胞抑制活性。与对照相比，每个视野的像元数减少了1.5倍。浓度为0.0288 mg / mL的绿茶提取物具有最明显的细胞毒性。暴露48小时后其表现正在增长。厚朴草提取物在较低浓度（0.0036 mg / mL）下表现出细胞毒活性。