Abstract—

Silver nanoparticles were synthesized using arabinogalactan and sodium dioctyl sulfosuccinate to reduce and stabilize them. The average hydrodynamic size of the nanoparticles, determined by photon correlation spectroscopy, was 30 nm, and the zeta potential was –34.04 ± 1.54 mV. According to the electron diffraction method, silver in the sol sample is in metallic form. The preparation of silver nanoparticles showed antibacterial activity against opportunistic Gram-negative (Escherichia coli) and Gram-positive (Bacillus subtilis and B. coagulans) bacteria. Silver nanoparticles also had antifungal activity against strains of phytopathogenic fungi of the genus Fusarium sporotrichioides and F. solani. A study of the cytotoxic activity of silver nanoparticles was made on human hepatoma cells of the HepG2 line. The inhibitory effect of silver nanoparticles on the metabolic activity and viability of tumor cells has been demonstrated. The average relative EC₅₀ values for silver nanoparticles were 1.5 ± 0.4 μg/ml and 41.2 ± 3.9 μg/mL. The preparation of stabilized silver nanoparticles can find application in medicine, as a potential antimicrobial and antitumor agent, as well as in agriculture as a means of suppressing growth of phytopathogenic fungi.

中文翻译：

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稳定化的银纳米粒子的制备及其对人肝癌HepG2细胞的抗菌和细胞毒活性研究

摘要—

使用阿拉伯半乳聚糖和磺基琥珀酸二辛酯钠合成银纳米颗粒，以还原和稳定它们。通过光子相关光谱法确定的纳米颗粒的平均流体动力学尺寸为30 nm，ζ电位为–34.04±1.54 mV。根据电子衍射法，溶胶样品中的银为金属形式。银纳米粒子的制备显示出对机会革兰氏阴性菌（大肠杆菌）和革兰氏阳性菌（枯草芽孢杆菌和凝结芽孢杆菌）的抗菌活性。银纳米颗粒还对镰孢镰刀菌（Fusarium sporotrichioides）和茄形镰刀菌（F.solani）的植物致病真菌菌株具有抗真菌活性。。对银纳米颗粒对HepG2系的人肝癌细胞的细胞毒性活性进行了研究。已经证明了银纳米颗粒对肿瘤细胞的代谢活性和生存能力的抑制作用。银纳米颗粒的平均相对EC ₅₀值为1.5±0.4μg/ mL和41.2±3.9μg/ mL。稳定化的银纳米颗粒的制备可以作为潜在的抗微生物剂和抗肿瘤剂在医学中应用，以及在农业中作为抑制植物病原性真菌生长的手段。