Natural rubber latex (NRL) is mainly used around traditional industrial products, but currently their target application is continuously expanding into tissue engineering. To broaden our knowledge of application in tissue engineering of NRL, we have focused on the surface modification of NRL nanoparticles through the biomineralization of hydroxyapatite (HA) using simulated body fluid in order to create a better cytocompatibility with controlled cell adhesion and mineralization properties in osteogenic culture to determine the effect on bone outcomes. Using MC3T3-E1 mouse osteoblastic-like cells incubated with NRL nanoparticles coated with HA layer, we have examined the osteogenic differentiation and expressions of multiple proteins and characteristic genes of mature osteoblast. We have successfully prepared the biocomposites composed of NRL and bone tissue.

天然胶乳（NRL）主要用于传统工业产品，但目前其目标应用正在不断扩展到组织工程中。为了拓宽我们在NRL组织工程中的应用知识，我们专注于通过使用模拟体液对羟基磷灰石（HA）进行生物矿化来对NRL纳米颗粒进行表面修饰，从而在成骨细胞中创建具有可控细胞粘附和矿化特性的更好的细胞相容性培养以确定对骨结局的影响。使用MC3T3-E1小鼠成骨样细胞与涂有HA层的NRL纳米颗粒孵育，我们检查了成熟成骨细胞的多种蛋白质和特征基因的成骨分化和表达。