Abstract—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expression is revealed in a significantly greater number of cells (from 25 to 50%). The dominant molecular phenotype (50–80% cells expressed both markers), the coexpression of vimentin and cytokeratins, is identified for the first time. The authors assume that the latter phenotype of melanoma cells may be a molecular marker of the tumor’s aggressiveness and metastatic potential.

中文翻译：

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波形蛋白和细胞角蛋白在人类黑素瘤细胞系中的共表达

摘要—使用流式细胞仪进行的双重免疫荧光分析揭示了七种人类转移性黑色素瘤细胞系中的分子异质性。据评估，相当少的细胞群体（最多10％）仅表达波形蛋白（黑色素瘤的主要标志物）。仅上皮细胞角蛋白表达在明显更多的细胞中显示（从25％到50％）。波形蛋白和细胞角蛋白的共表达是首次鉴定出主要的分子表型（50–80％的细胞同时表达两种标记）。作者认为，黑色素瘤细胞的后一种表型可能是肿瘤侵袭性和转移潜力的分子标记。