The present research investigates the interactive effects of 24-epibrassinolide (0, 0.02 and 0.5 µM) plus thiamine (0 and 200 µM) treatments on some of physio-biochemical parameters in Brassica napus plants under Cadmium Chloride toxic levels; 250 and 500 µM. Application of 24-epibrassinolide 0.02 µM plus thiamine 200 µM significantly increased growth and biochemical parameters such as shoot length, shoot dry weight and total chlorophyll content compared to the Cd stress conditions. While, reduced oxidative markers such as malondialdehyde, hydrogen peroxide contents and leaves Cd accumulation. Apply of 24-epibrassinolide plus thiamine increased non-enzymatic antioxidant contents and activity of antioxidant enzymes such as catalase, ascorbate peroxidase and guaiacol peroxidase. Also, enhancing the DPPH radical scavenging potential along with increasing PAL activity indicates the major influence of 24-epibrassinolide 0.02 µM and thiamine 200 µM in the reduction of Cd-induced oxidative stress in canola plants. Also In the present of 250 and 500 µM Cd, EBL 0.02 μM plus thiamine 200 μM significantly decreased H₂O₂ accumulation by 49% and 38% compared to 250 and 500 µM Cd treatment without applying the plant growth regulators respectively. Generally, Application of EBL 0.02 μM plus thiamine 200 μM improved the oxidative resistance of Canola plant under cadmium stress 250 µM.

本研究调查了24-表油菜素内酯（0、0.02和0.5 µM）和硫胺素（0和200 µM）处理对甘蓝型油菜某些生理生化参数的相互作用。氯化镉毒性水平下的植物；250和500 µM。与Cd胁迫条件相比，施用0.02 µM的24-表油菜素内酯加200 µM的硫胺素可显着提高生长和生化参数，例如苗长，苗干重和总叶绿素含量。同时，减少了氧化标记，如丙二醛，过氧化氢的含量，并使Cd积累。使用24-表油菜素内酯加硫胺素可增加非酶类抗氧化剂的含量，并提高抗氧化酶（如过氧化氢酶，抗坏血酸过氧化物酶和愈创木酚过氧化物酶）的活性。另外，提高DPPH自由基清除能力以及提高PAL活性表明，在芥花籽油中，24-epbrassinolide 0.02 µM和硫胺素200 µM降低了Cd诱导的氧化胁迫。与不使用植物生长调节剂的250和500 µM Cd处理相比，₂ O ₂累积分别增加了49％和38％。通常，使用EBL 0.02μM加硫胺素200μM可以改善Canola植物在250μM镉胁迫下的抗氧化性。