In the present study, the cDNA (designated MuPR1) of PR1 gene was obtained from mulberry. Our results showed that MuPR1 shares some conserved characteristics with its homologues from different plant families. MuPR1 was localized exclusively to the extracellular spaces, and the MuPR1 gene was expressed constitutively in all selected mulberry tissues, although its expression levels varied substantially. It was also showed that MuPR1 expression was induced by pathogen, MeJA, SA and GA₃, and transgenic Arabidopsis overexpressing MuPR1 showed enhanced resistance to Botrytis cinerea and Pst. DC3000. Moreover, the peptide derived from MuPR1 protein C-terminus with a PxGNxxxxxPY motif was suggested to play a role in activating plant resistance. Furthermore, the MuPR1 gene may have roles in mediating the rates of oxygen radical formation and detoxification. Therefore, the MuPR1 gene may be a candidate gene for breeding resistant mulberry varieties in the future.

在本研究中，PR1基因的cDNA（称为MuPR1）是从桑树中获得的。我们的结果表明，MuPR1与来自不同植物科的同源物具有一些保守的特性。MuPR1仅定位于细胞外空间，并且MuPR1基因在所有选定的桑树组织中组成性表达，尽管其表达水平差异很大。还显示出MuPR1表达被病原体，MeJA，SA和GA ₃诱导，并且过表达MuPR1的转基因拟南芥显示出对灰葡萄孢和Pst的增强的抗性。DC3000。此外，有人提出具有PxGNxxxxxPY基序的MuPR1蛋白C末端衍生的肽在激活植物抗性中起作用。此外，MuPR1基因可能在介导氧自由基形成和排毒的速率中发挥作用。因此，MuPR1基因可能是将来育种抗性桑树品种的候选基因。