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Molecular characterisation of nerol dehydrogenase gene (PmNeDH) from Persicaria minor in response to stress-related phytohormones
Journal of Plant Interactions ( IF 3.2 ) Pub Date : 2019-08-05 , DOI: 10.1080/17429145.2019.1638457
Cheng Seng Tan 1 , Nur-Athirah Abd-Hamid 2 , Jin Kiat Chew 2 , Maizom Hassan 2 , Ismanizan Ismail 1, 2 , Chyan Leong Ng 2 , Zamri Zainal 1, 2
Affiliation  

Citral is a mixture of neral and geranial, which are of great interest to the fragrance industry due to its lemon-scented aroma. A newly characterized nerol dehydrogenase of Persicaria minor (PmNeDH) from our recent findings has shown a capacity to convert citral from nerol. Differential gene expression analysis revealed that the expression level of PmNeDH was highly upregulated during early treatment of several stress-related phytohormones i.e. methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA). SA and ABA were shown to have a prolonged effect on PmNeDH expression level until second day of treatment. The findings were in agreement with the cis-regulatory elements predicted from the gene promoter. The phylogenetic relationship of PmNeDH with its homologs from the medium-chain dehydrogenases/reductases (MDR) superfamily was also mentioned. In this study, we proposed a possible biological function of PmNeDH gene in P. minor, which might play significant roles in plant defence mechanism.



中文翻译:

波斯菊神经醇脱氢酶基因(PmNeDH)的分子表征对应激相关植物激素的响应

柠檬醛是中草药和香叶醛的混合物,由于其柠檬香气,在香水行业引起了极大的兴趣。根据我们最近的发现,一种新鉴定的小Persicaria小鸟的神经醇脱氢酶(PmNeDH)已显示出将柠檬醛转化为神经醇的能力。差异基因表达分析表明,PmNeDH的表达水平在几种与应激有关的植物激素(茉莉酸甲酯(MeJA),水杨酸(SA)和脱落酸(ABA))的早期治疗中高度上调。在治疗第二天之前,SA和ABA对PmNeDH表达水平有延长的影响。研究结果与从基因启动子预测的调控元件。还提到了PmNeDH与中链脱氢酶/还原酶(MDR)超家族同源物的系统发育关系。在这项研究中,我们提出的一个可能的生物学功能PmNeDH基因P.未成年人,这可能会在植物防御机制发挥显著作用。

更新日期:2019-08-05
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