The present study was designed to assess the fluoride mediated antioxidant defence responses in Spirodela polyrhiza, grown under hydroponic conditions. The plants of S. polyrhiza were exposed to different fluoride concentrations (0, 5, 10, 15, 20, 25, 50 ppm) for the time period 24, 72, 120 and 168 h. Fluoride was observed to be readily accumulated by S. polyrhiza at all the exposure periods. Increased accumulation of fluoride triggered the oxidative stress that was quite evident from enhanced electrolyte leakage, proline, anthocyanin and phenolic content To counterbalance the stress, plant responded by the alterations in the activities of antioxidative enzymes (SOD, CAT, APOX, POD, GR), that was also supposed to confer tolerance ability to plant. Moreover, SEM-EDX, CLSM and FT-IR studies were also performed to confirm oxidative damage. Hence, it can be elucidated that this plant can act as an efficient fluoride accumulator and antioxidant defence system minimized fluoride toxicity.

本研究旨在评估在水培条件下生长的螺旋藻中氟化物介导的抗氧化防御反应。在24、72、120和168小时的时间段内，多叶链霉菌的植物暴露于不同的氟化物浓度（0、5、10、15、20、25、50 ppm）。观察到氟化物很容易被沙门氏菌积累。在所有暴露时期。氟化物积累的增加触发了氧化应激，这从电解质泄漏，脯氨酸，花色苷和酚含量的增加中可以明显看出。为了平衡胁迫，植物通过抗氧化酶（SOD，CAT，APOX，POD，GR）活性的变化来应对，这也应该赋予植物耐性。此外，还进行了SEM-EDX，CLSM和FT-IR研究以确认氧化损伤。因此，可以阐明该植物可以作为有效的氟化物蓄积器，而抗氧化剂防御系统可将氟化物的毒性降至最低。