Hydrogen sulfide (H₂S) is a highly diffusible, gaseous molecule that has been proved to control a range of physiological responses in many plant stresses. Exposure to UV-B radiation leads to the generation of reactive oxygen species that cause oxidative damages. In this study, sodium hydrosulfide (NaHS) was used as a H₂S donor for the alleviation of damage caused by UV-B in Borage plant. Lipid peroxidation, protein oxidation, and H₂O₂ content were increased in plant exposed to UV-B. Results showed that ascorbate peroxidase, guaiacol peroxidase, and catalase activities and reduced glutathione (GSH) did not change significantly in stressed plants when compared with control, but flavonoids concentration was increased considerably. Pre-treatment of plants with NaHS (120 μM), significantly decreased the Malondialdehyde content, carbonyl groups, and H₂O₂. Activities of ascorbate peroxidase and guaiacol peroxidase enzymes increased in plants which were pre-treated while catalase activity decreased. The reduced glutathione content increased in borage plant leaves. HPLC analysis of flavonoids demonstrated that pre-treatment with NaHS caused a decrease in flavonoid content. These findings suggest that H₂S alleviates UV-B damages may occur through enhancement of antioxidative enzyme activities and GSH content in borage seedlings.

硫化氢（H ₂ S）是一种高度扩散的气态分子，已被证明可以控制许多植物胁迫下的一系列生理反应。暴露于UV-B辐射会导致产生活性氧，从而导致氧化损伤。在这项研究中，使用硫化氢钠（NaHS）作为H ₂ S供体，以减轻琉璃苣工厂中UV-B造成的损害。脂质过氧化，蛋白质氧化和H ₂ O ₂暴露于UV-B的植物中含量增加。结果表明，与对照相比，抗逆植物中抗坏血酸过氧化物酶，愈创木酚过氧化物酶和过氧化氢酶的活性以及谷胱甘肽减少（GSH）没有明显变化，但黄酮类化合物的浓度却显着增加。用NaHS（120μM）预处理植物，可显着降低丙二醛含量，羰基和H ₂ O ₂。预处理过的植物中抗坏血酸过氧化物酶和愈创木酚过氧化物酶的活性增加，而过氧化氢酶的活性则下降。琉璃苣植物叶片中降低的谷胱甘肽含量增加。黄酮类化合物的HPLC分析表明，用NaHS预处理可降低类黄酮含量。这些发现表明H ₂S减轻琉璃苣幼苗中抗氧化酶活性和谷胱甘肽含量的增加，可能减轻UV-B的损害。