Mutated COX-2 has become the new molecular marker of aspirin resistance. However, there is still a technical ‘bottleneck’ for direct and sensitive detection of circulating COX-2 mutant gene. In this work, we reported a simple and ultrasensitive electrochemical method for COX-2–765G/C (rs20417) detection for the first time. Polyallylamine (PAA) functionalised Pt nanostructures with long-spined sea urchin-like morphology (Pt-LSSUs@PAA) was synthesised by a simple chemical method for the construction of nano-sensing interface. Ru(NH₃)₆³⁺ is used as a primary electron acceptor that is electrostatically attracted to peptide nucleic acid modified electrodes and Fe(CN)₆³⁻ is introduced into the redox system as secondary electron acceptor to regenerate Ru³⁺ after electrochemical reduction for multiple redox cycles. Different pulse voltammetry was applied to record the electrochemical signals. Under optimal conditions, the DNA sensors showed a wide linear relationship, from 10 fM to 1 nM, with detection limits of 3.3 fM (S/N = 3). This study will provide the basis for the precise use of aspirin, and it has important guiding value for individual drug testing of cardiovascular disease.

突变的COX-2已成为阿司匹林耐药性的新分子标记。但是，仍然存在直接和灵敏地检测循环COX-2突变基因的技术“瓶颈”。在这项工作中，我们首次报道了一种用于COX-2–765G / C（rs20417）检测的简单且超灵敏的电化学方法。通过简单的化学方法合成了具有长旋海胆状形态（Pt-LSSUs @ PAA）的聚烯丙胺（PAA）功能化的Pt纳米结构。Ru（NH ₃）₆ ³⁺用作静电被肽核酸修饰电极和Fe（CN）₆ ^3-吸引的初级电子受体将其作为二次电子受体引入氧化还原体系中，以在多个还原周期进行电化学还原后再生Ru ³⁺。应用不同的脉冲伏安法记录电化学信号。在最佳条件下，DNA传感器显示出从10 fM到1 nM的宽线性关系，检测极限为3.3 fM（S / N = 3）。这项研究将为精确使用阿司匹林提供基础，并且对心血管疾病的单独药物检测具有重要的指导价值。