Following the idea of green chemistry, especially green analytical chemistry, a series of stationary phases was synthesized. The obtained materials connect polar and hydrophobic groups in the structure of bonded ligands. These specific surface properties provide the stability of the stationary phase in pure water as a mobile phase. To confirm the solvation ability in purely aqueous mobile phases, excess isotherms of water and acetonitrile were determined. Further, the mixtures of nucleosides, nucleic bases and purine alkaloids were applied to test the separation selectivity of stationary phases in purely aqueous conditions at ambient temperature without any additives to the mobile phase. Among the four tested stationary phases, it is possible to find one for separation of each group of analytes that offers selective separation in reasonable time. The presented data confirms that it is possible to synthesize stationary phases for the separation of target mixtures in pure water conditions.

遵循绿色化学，特别是绿色分析化学的思想，合成了一系列固定相。所获得的材料在键合的配体的结构中连接极性和疏水基团。这些特定的表面性质为纯水作为流动相提供了固定相的稳定性。为了确认在纯水流动相中的溶剂化能力，测定了水和乙腈的过量等温线。此外，将核苷，核酸碱基和嘌呤生物碱的混合物用于在环境温度下在纯水条件下测试固定相的分离选择性，而对流动相没有任何添加剂。在四个经过测试的固定相中，可以找到一种用于分离每组分析物的方法，这些分析物可在合理的时间内提供选择性的分离。