In this study, a novel strategy was established to produce diosgenin from Discorea nipponica Makino tubers, in which pressurized biphase acid hydrolysis and Soxhlet extraction of total saponins were incorporated for the first time. The hydrolysis conditions were optimized by response surface methodology after the preliminary investigation on affecting factors by single-factor experiments and L₁₆(4⁵) orthogonal experiments. Under the optimal conditions, namely dioscin (eq. 8 g of the DNM tubers) was hydrolyzed in a biphase system composed of 50 mL of 6 μL/mL H₂SO₄ solution and 50 mL of petroleum ether (90–120°C) at 140°C for 2 h, the highest yield of diosgenin has been achieved at 1.87%, that was 85.1% higher than the conventional method while the H₂SO₄ consumption reduced by 92.5%. The results demonstrated that the newly developed approach was more effective and cleaner, and promising value-added technology for the extraction of diosgenin from DNM tubers in industrial application.

在这项研究中，建立了一种新的策略来从Discorea nipponica Makino块茎中生产薯os皂苷元，其中首次采用了加压双相酸水解和总皂甙的索氏提取法。在通过单因素实验和L ₁₆（4 ⁵）正交实验对影响因素进行初步研究后，通过响应面法优化了水解条件。在最佳条件下，即在由50 mL 6μL/ mL H ₂ SO ₄组成的双相系统中，将薯os皂苷（当量为8 g的DNM块茎）水解。溶液和50 mL石油醚（90–120°C）在140°C下2 h，薯os皂素的最高收率为1.87％，比传统方法高85.1％，而H ₂ SO _4的消耗量减少了92.5％。结果表明，新开发的方法更有效，更清洁，并且有望在工业应用中从DNM块茎中提取薯os皂苷元的增值技术。