The aim of the present study is to develop an eco-friendly, simple, cost-effective and single-step method for the synthesis of gold nanoparticles using Agrimonia pilosa (AP-AuNPs) aerial parts extract and evaluation of their antioxidant and cytotoxic activity. Synthesis of AP-AuNPs was observed by a color change from light yellow to wine-red, and confirmed by the UV–VIS absorbance peak at 535 nm. From transmission electron microscopic analysis it is observed that the AP-AuNPs were hexagonal with size of about 20–50 nm. The field emission-scanning electron microscopy image revealed the synthesized AP-AuNPs to have a hexagonal shape. The energy-dispersive X-ray and X-ray diffraction analyses confirmed the metal gold and crystalline nature of AP-AuNPs. A. pilosa extract-mediated AP-AuNPs were found to be non-toxic on normal cells (HaCaT, Keratinocyte), exhibited a concentration-dependent cytotoxic effect on cancer cells (AGS, gastric adenocarcinoma) and antioxidant potential. The AP-AuNPs are synthesized using the A. pilosa aerial part extract using a simple and eco-friendly method. The synthesized AP-AuNPs can be useful in therapeutics.

本研究的目的是开发一种生态友好，简单，具有成本效益的单步方法，该方法用于使用松毛龙牙草（AP-AuNPs）地上部分提取物合成金纳米颗粒，并评估其抗氧化和细胞毒性活性。从浅黄色到酒红色的颜色观察到了AP-AuNPs的合成，并在535 nm处的UV-VIS吸收峰证实了这一点。从透射电子显微镜分析可以看出，AP-AuNPs是六边形的，大小约为20-50 nm。场发射扫描电子显微镜图像显示合成的AP-AuNP具有六边形形状。能量色散X射线和X射线衍射分析证实了AP-AuNPs的金属金和晶体性质。A.草提取物介导的AP-AuNPs对正常细胞（HaCaT，角质形成细胞）无毒，对癌细胞（AGS，胃腺癌）表现出浓度依赖性的细胞毒性作用和抗氧化能力。AP-AuNPs是使用一种简单且生态友好的方法，使用A. pilosa空中部分提取物合成的。合成的AP-AuNPs可用于治疗。