The environment-friendly disposal approaches for sewage sludge remain a challenge worldwide, and agricultural application of the treated sewage sludge, i.e. biosolids, as soil conditioner and nutrient source for plants, is still one of the most promising options for dealing with the waste. In this study, we compared the effects of long-term applications of biosolids and mineral fertilizer on soil microbial community using phospholipid fatty acid profiles in an agricultural field. The microbes predominant in the mineral fertilizer soil remained the most abundant in the biosolids-amended soil. Bacterial and fungal communities presented downtrends in both absolute and relative amounts; however, percentage of Gram-positive bacteria remained unchanged. Soil pH displayed significant negative correlations with microbial communities and explained 64%, 56% and 44% of the variances in microbial biomass, bacteria and fungi, respectively, while soil NO₃^–-N was positively correlated with microbial biomass, bacteria and fungi (r² = 0.53, 0.51 and 0.54, respectively).

污水污泥的环境友好处置方法仍然是世界范围内的挑战，处理过的污水污泥，即生物固体，作为土壤调节剂和植物养分来源，在农业上的应用仍然是处理垃圾的最有希望的选择之一。在这项研究中，我们比较了在农田中使用磷脂脂肪酸谱对长期施用生物固体和矿物肥料对土壤微生物群落的影响。矿物肥料土壤中占优势的微生物在生物固体改良土壤中仍然最丰富。细菌和真菌群落的绝对和相对数量均呈下降趋势。然而，革兰氏阳性细菌的百分比保持不变。土壤pH与微生物群落呈显着负相关，解释为64％，₃ ^– -N与微生物量，细菌和真菌呈正相关（r ² 分别为0.53、0.51和0.54）。