Soil potential nitrification rate (PNR) and diversity of ammonia-oxidizing microbes were investigated in spiked soils with Cu and Sulfadiazine (SDZ). An obvious decrease of PNR was observed with the increase of Cu and SDZ concentrations in the soil. Real-time fluorescence quota PCR result showed that AOA and AOB were slightly stimulated at the gene level in both contaminated soils. Sequential analysis indicated that 200 mg kg⁻¹ Cu could improve AOA diversity but reduce AOB diversity, but 5 mg kg⁻¹ SDZ caused a decrease of both AOA and AOB diversity. Microbial community’s analysis also found that 200 mg kg⁻¹ Cu and 5 mg kg⁻¹ SDZ had different influence on the populations of AOA and AOB. It could be concluded that Cu and SDZ might have a different ecological effect mechanism on soil potential nitrification and ammonia-oxidizing microbial communities.

研究了加标土壤中铜和磺胺嘧啶（SDZ）的土壤潜在硝化率（PNR）和氨氧化微生物的多样性。随着土壤中Cu和SDZ浓度的增加，PNR明显降低。实时荧光定量PCR结果表明，两种污染土壤在基因水平上均对AOA和AOB有轻微的刺激作用。顺序分析表明200 mg kg ^-1 Cu可以改善AOA多样性，但会降低AOB多样性，但是5 mg kg ^-1 SDZ会导致AOA和AOB多样性降低。微生物界的分析还发现200 mg kg ^-1铜和5 mg kg ^-1SDZ对AOA和AOB种群的影响不同。可以得出结论，铜和SDZ可能对土壤潜在的硝化作用和氨氧化微生物群落具有不同的生态效应机制。