Free enzymes employed as biological catalysts have many advantages such as low reaction time, involving low energy and less waste output when compared to conventional chemical catalysts. However, commercial utilization of free enzymes is often hampered by the lack of operational stability, high cost, and non‐reusability. Immobilization of enzyme is an option to overcome these obstacles. Immobilized enzyme expresses stable performance in organic solvents even in adverse pH, which makes the biomolecules reusable and prosperous as a biological catalyst. Biological catalysis with immobilized enzymes is found to be an alternative method instead of chemical catalysis for chemical reactions in the foreseeable future. Sources of lipase, techniques in immobilization and cross‐linkers are dealt with in this paper.

中文翻译：

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脂肪酶的固定化-评论。第一部分：酶的固定化

与常规化学催化剂相比，用作生物催化剂的游离酶具有许多优势，例如反应时间短，能耗低和废物输出少。但是，由于缺乏操作稳定性，成本高和不可重复使用性，游离酶的商业利用常常受到阻碍。固定化酶是克服这些障碍的一种选择。固定化酶即使在不利的pH值下也能在有机溶剂中表现出稳定的性能，这使得生物分子可重复使用并作为生物催化剂而繁荣。在可预见的将来，发现用固定化酶进行生物催化是代替化学催化的另一种方法。本文讨论了脂肪酶的来源，固定化技术和交联剂。