Attempts based on increasing the efficacy of Baculovirus and/or reducing the application concentration of synthetic insecticides through integrated lepidopteran management are appreciated role for conserving the environment. Impact of the multiple nucleopolyhedrosis virus (SpliMNPV) with emamectin benzoate (Em) against the cotton leaf worm, Spodoptera littoralis, was examined to identify the effective strategy for applying both agents in the control program successfully. The LC50 and LC90 were drastically decreased from 1.9 × 106 and 1.0 × 1010 PIB/ml in SpliMNPV treatment to reach 8.87 × 101 and 1 × 104 PIB/ml, respectively in the SpliMNPV concentrations + Em LC25 treatment. This interaction was considered as potentiation. Larvicidal activity of Em was highly increased by Em concentrations + SpliMNPV LC25 treatment than the separately Em treatment; however, this interaction was considered as additive. Moreover, the mixture treatment (SpliMNPV LC99 + Em LC50) provided almost full protection of viral pathogenicity up to 48 h at natural exposure periods. Furthermore, the mixture treatment had a negative impact on the insect survival and reproduction of treated individuals. Results indicated that the virus infectivity was increased by a mixture treatment of SpliMNPV + Em in particular facing UV sunlight, which causes virus degradation as well as reduced the effective doses of Em. These findings suggest that this simultaneous treatment maybe an effective technique to be applied in S. littoralis control strategy.

中文翻译：

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苯甲酸依莫菌素对滨海斜纹夜蛾核多角体病毒感染性的影响（鳞翅目：夜蛾科）

基于杆状病毒的功效和/或通过鳞翅目动物的综合治理降低合成杀虫剂的使用浓度而进行的尝试被认为对保护环境具有重要作用。研究了含多聚苯甲酸酯（Em）的多核多角体病毒（SpliMNPV）对棉叶蠕虫Spodoptera littoralis的影响，以确定在控制程序中成功应用两种药剂的有效策略。在SpliMNPV浓度+ Em LC25处理中，LC50和LC90分别从SpliMNPV处理中的1.9×106和1.0×1010 PIB / ml显着降低，分别达到8.87×101和1×104 PIB / ml。这种相互作用被认为是增强作用。与单独的Em处理相比，Em浓度+ SpliMNPV LC25处理可大大提高Em的杀幼虫活性。但是，这种相互作用被认为是加性的。此外，混合处理（SpliMNPV LC99 + Em LC50）在自然暴露时间长达48小时内几乎可以完全保护病毒致病性。此外，混合处理对被处理个体的昆虫存活和繁殖具有负面影响。结果表明，通过SpliMNPV + Em的混合处理（特别是面对紫外线）可提高病毒的感染性，这会导致病毒降解并降低Em的有效剂量。这些发现表明，这种同时治疗可能是一种有效的技术，可用于滨海链球菌的控制策略。此外，混合处理（SpliMNPV LC99 + Em LC50）在自然暴露时间长达48小时内几乎可以完全保护病毒致病性。此外，混合处理对被处理个体的昆虫存活和繁殖具有负面影响。结果表明，通过SpliMNPV + Em的混合处理（特别是面对紫外线）可提高病毒的感染性，这会导致病毒降解并降低Em的有效剂量。这些发现表明，这种同时治疗可能是一种有效的技术，可用于滨海链球菌的控制策略。此外，混合处理（SpliMNPV LC99 + Em LC50）在自然暴露时间长达48小时内几乎可以完全保护病毒致病性。此外，混合处理对被处理个体的昆虫存活和繁殖具有负面影响。结果表明，通过SpliMNPV + Em的混合处理（特别是面对紫外线），病毒的感染力增加，这会导致病毒降解并降低Em的有效剂量。这些发现表明，这种同时治疗可能是一种有效的技术，可用于滨海链球菌的控制策略。混合处理对被处理个体的昆虫存活和繁殖有负面影响。结果表明，通过SpliMNPV + Em的混合处理（特别是面对紫外线），病毒的感染力增加，这会导致病毒降解并降低Em的有效剂量。这些发现表明，这种同时治疗可能是一种有效的技术，可用于滨海链球菌的控制策略。混合处理对被处理个体的昆虫存活和繁殖有负面影响。结果表明，通过SpliMNPV + Em的混合处理（特别是面对紫外线），病毒感染力增加，这会导致病毒降解并降低Em的有效剂量。这些发现表明，这种同时治疗可能是一种有效的技术，可用于滨海链球菌的控制策略。