This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMCs) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMCs from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control. The cytotoxicity was determined by trypan blue exclusion method and IL-1β secretion by ELISA. TNF-α, IL-6, and IL-8 secretions were measured using Bioplex Multiplex Immunoassay. Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMCs than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains (JP2 and JP2-like) induced a higher response than all other strains. When combined, Lactobacillus significantly reduced the toxicity and the IL-1β secretion induced by A. acinomycetemcomitans. The effect varied between the subtypes and the reduction was highest for the JP2 and JP2-like strains. The Lactobacillus paracasei strain SD1 had a higher reducing effect than the other Lactobacillus strains. This strain had a consistent reducing effect on all subtypes of A. actinomycetemcomitans cytotoxicity, and release of IL-1β, IL-6, IL-8, and TNF-α from PBMCs of the blood donors. A strong and significant variation in cytokine release between the six blood donors was noticed. Lactobacillus spp. and L. paracasei SD1 in particular, showed a limited but statistically significant reducing interaction with A. actinomycetemcomitans toxicity and release of cytokines in vitro.

中文翻译：

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口服乳酸杆菌菌株可降低体外暴露于聚集放线菌亚型的外周血单核细胞的细胞毒性和细胞因子释放。

本研究评估了口服乳酸杆菌对外周血单核细胞 (PBMC) 在体外暴露于聚集放线菌亚型时对细胞毒性和细胞因子释放的影响。使用了代表不同亚型的八种伴放线放线菌菌株和三种乳杆菌菌株的上清液和细胞壁提取物 (CWE)。来自六名献血者的 PBMC 暴露于单独或组合放线菌或乳酸杆菌菌株的上清液和 CWE，未处理的细胞作为对照。细胞毒性通过台盼蓝排除法和 IL-1β 分泌通过 ELISA 确定。使用 Bioplex Multiplex Immunoassay 测量 TNF-α、IL-6 和 IL-8 分泌物。所有细菌菌株的上清液或 CWE 均显示出细胞毒性和 IL-1β 分泌以及 A. 与乳酸杆菌菌株相比，伴放线菌对 PBMC 的影响通常显着更高。两种剧毒放线放线菌菌株（JP2 和 JP2 样）比所有其他菌株诱导更高的反应。当组合时，乳酸杆菌显着降低了 A. acinomycetemcomitans 诱导的毒性和 IL-1β 分泌。亚型之间的效果不同，JP2 和 JP2 样菌株的减少量最高。副干酪乳杆菌菌株 SD1 比其他乳杆菌菌株具有更高的还原效果。该菌株对伴随放线放线菌的所有亚型的细胞毒性以及从献血者的 PBMC 中释放 IL-1β、IL-6、IL-8 和 TNF-α 具有一致的降低作用。注意到六名献血者之间细胞因子释放的强烈而显着的变化。乳酸菌属 尤其是副干酪乳杆菌 SD1，在体外与放线菌毒性和细胞因子释放的相互作用有限，但具有统计学意义。