During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalogue of 2'-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.

中文翻译：

---

斑马鱼发育中核糖体从早期类型到晚期类型的转变涉及 rRNA 2'-O-Me 位点子集的变化

在斑马鱼发育过程中，早期类型的 rRNA 逐渐被在序列上有很大差异的晚期类型所取代。我们从发育阶段将 RiboMeth-seq 应用于 rRNA 以分析 2'-O-Me，以了解甲基化模式的变化是否是转变的一个组成部分。我们编制了 2'-O-Me 位点和同源框 C/D 引导 RNA 的目录，其中包含 98 个高置信度位点，其中包括 10 个其他脊椎动物未知的位点，其中一个位点特定于晚期型 rRNA。我们确定了在开发过程中甲基化状态发生变化的一组位点，并发现其中一些可以通过其同源 SNORD 的可用性来解释。在斑马鱼中发现的新位点丰富了在发育过程中发生变化的位点。