Species of Dipsacus have been widely used in folk medicine for their neuroprotective, antiosteoporotic, antioxidative, anticomplementary, and antibacterial activities. However, there has been but a limited amount of research on the anticancer effect of one of the most popular representatives of this genus, D. fullonum. Also, the cytotoxic activity has not yet been investigated of the constituents of D. fullonum leaves. The purpose of this study was to evaluate the cytotoxic activity of the bis-iridoid glycosides isolated from D. fullonum leaves against murine fibroblast NIH/3T3, mouse melanoma B16F10, HeLa human cervical cancer, human breast cancer MCF7 and MDB-MB-231 cells. The bis-iridoids, obtained by chromatographic fractionation of the extract of D. fullonum leaves, were characterized by thin-layer chromatography and high-performance liquid chromatography-mass spectrometry (HPLC-MS)/MS analysis. The cytotoxicity of the iridoid fraction was evaluated by WST-1 assay, and the number of dead cells was determined by the propidium iodide test. HPLC-MS/MS analysis showed the isolated bis-iridoid fraction to consist mainly of sylvestroside III and/or sylvestroside IV. This fraction was applied to cell cultures and kept for 48 and 72 hours. The results demonstrated that the iridoid glycosides had a differential ability to induce cell death in normal and cancer cells. The study confirmed that the bis-iridoids extracted from D. fullonum leaves had a selective cytotoxic effect on human breast cancer cell lines MCF7 and MDB-MD-231, while their cytotoxic effect on noncancer cells was low.

Dipsacus种类因其神经保护，抗骨质疏松，抗氧化，抗补体和抗菌活性而被广泛用于民间医学。然而，关于该属中最流行的代表之一，D.fullonum的抗癌作用的研究还很有限。另外，尚未研究满枝杜鹃叶的成分的细胞毒性活性。这项研究的目的是评估从满满的D.fullonum分离的双-iridoid糖苷的细胞毒活性对鼠成纤维细胞NIH / 3T3，小鼠黑素瘤B16F10，HeLa人宫颈癌，人乳腺癌MCF7和MDB-MB-231细胞具有抗性。通过对D. fullonum的提取物进行色谱分离得到的双虹膜类化合物通过薄层色谱和高效液相色谱-质谱（HPLC-MS）/ MS分析对叶片进行表征。通过WST-1测定法评估了虹膜状组分的细胞毒性，并通过碘化丙啶试验确定了死细胞的数量。HPLC-MS / MS分析显示，分离的双-类虹彩馏分主要由西维他糖苷III和/或西维他糖苷IV组成。将该级分应用于细胞培养，并保持48和72小时。结果表明，环烯醚酮苷在正常细胞和癌细胞中具有诱导细胞死亡的不同能力。研究证实，从满枝D中提取的双虹膜类化合物 叶对人乳腺癌细胞MCF7和MDB-MD-231具有选择性的细胞毒性作用，而对非癌细胞的细胞毒性作用却很低。