Salvia miltiorrhiza Bge. (Danshen) is widely used to improve blood circulation and the dredge meridian in traditional Chinese medicine. In the present study, we evaluated the effects of dihydrotanshinone I (DHTS), a natural product from Danshen, on chronic high-fat feeding-induced cardiac remodeling and dysfunction. DHTS (25 mg/kg, intraperitoneal) did not affect blood glucose, insulin levels, and glucose intolerance. However, it alleviated diastolic dysfunction induced by the high-fat diet, as indicated by the increase in the ratio of peak early filling velocity to peak late filling velocity of the mitral and suppression of the extension of the isovolumic relaxation phase of the left ventricle. Further investigations revealed that DHTS ameliorated high-fat induced cardiac hypertrophy in mice and suppressed insulin-induced enlargement of cardiomyocytes in vitro. In neonatal cardiomyocytes, DHTS restored insulin-induced suppression of CCAAT/enhancer-binding protein beta-2 isoform (CEBPβ) and the phosphorylation of glycogen synthase kinase-3β (GSK3β) and extracellular signal-regulated kinase (ERK). Taken together, our results indicated that DHTS ameliorated cardiac hypertrophy and diastolic dysfunction in high-fat-fed mice, probably through the inhibition of insulin-induced suppression of CEBPβ and phosphorylation of GSK3β and ERK in cardiomyocytes.

丹参哎呀 （丹参）被广泛用于改善血液循环和中医疏通经络。在本研究中，我们评估了丹参的天然产物二氢丹参酮I（DHTS）对慢性高脂喂养引起的心脏重塑和功能障碍的影响。DHTS（25 mg / kg，腹膜内）不影响血糖，胰岛素水平和葡萄糖耐量。然而，它减轻了高脂饮食引起的舒张功能障碍，这表现为二尖瓣的早期早期充盈速度与晚期晚期充盈速度之比的增加和抑制左心室等容舒张期的延长。进一步的研究表明，DHTS可以减轻高脂诱导的小鼠心肌肥大，并在体外抑制胰岛素诱导的心肌细胞肥大。在新生儿心肌细胞中，DHTS恢复了胰岛素诱导的CCAAT /增强子结合蛋白β-2亚型（CEBPβ）的抑制以及糖原合酶激酶3β（GSK3β）和细胞外信号调节激酶（ERK）的磷酸化。综上所述，我们的结果表明，DHTS可能通过抑制胰岛素诱导的心肌细胞CEBPβ抑制和GSK3β和ERK磷酸化而改善了高脂喂养小鼠的心脏肥大和舒张功能障碍。