The purpose of this article is to establish a method to use ultra-high performance liquid chromatography (UPLC)-mass spectrometry (MS)/MS to simultaneously determine 9 main components of Lonicera japonica Thunb. in negative-ion scanning mode, and the main components were analyzed by chemometrics. The chromatographic separation uses the Thermo Hypersil GOLD column (100 mm × 2.1 mm, 1.9 µm) with a constant temperature of 45 °C. The mobile phase consists of methanol and water containing 0.2% formic acid. The results show that 9 compounds had a good linear relationship (R² > 0.9991), and both intraday and interday precisions and stability have the eligible ranges of relative SDs (RSDs; 0.96%-2.26%, 0.52%-3.04%, and 0.85%-2.15%, respectively). The recovery rates were between 75.90% and 110.58%. The results of chemometrics including hierarchical cluster analysis and principal component analysis showed that there were obvious differences in the content of active components in L. japonica from different regions, and the compounds with the highest contribution to the drug were identified. Through the UPLC-MS/MS combined chemometrics analysis of L. japonica, this experiment can provide a reference for further research on the modernization and innovation of L. japonica and the application research of a high level and multidirection.

本文的目的是建立一种使用超高效液相色谱（UPLC）-质谱（MS）/ MS同时测定忍冬忍冬的9种主要成分的方法。在负离子扫描模式下，通过化学计量学对主要成分进行了分析。色谱分离使用Thermo Hypersil GOLD色谱柱（100 mm×2.1 mm，1.9 µm），恒定温度为45°C。流动相由甲醇和含0.2％甲酸的水组成。结果表明，9种化合物具有良好的线性关系（R²> 0.9991），并且日内和日间的精度和稳定性均具有相对SD的合格范围（RSD；分别为0.96％-2.26％，0.52％-3.04％和0.85％-2.15％）。回收率在75.90％至110.58％之间。化学计量学的结果包括层次聚类分析和主成分分析表明，不同地区的粳稻中活性成分含量存在明显差异，并且鉴定出对药物的贡献最大的化合物。通过对粳稻的UPLC-MS / MS化学计量学分析，该实验可为进一步研究粳稻的现代化和创新以及高水平，多方向的应用研究提供参考。