Endemic goitre and associated iodine deficiency disorders (IDDs) are a major concern in public health even in the period of post salt iodization in many regions. Among others the consumption of cyanogenic plants found responsible for the persistence of such diseases. Bamboo shoots (BS) is one such cyanogenic plant food that caused disruption of certain thyroid hormone synthesizing regulatory element as has already been reported in our earlier study. In this investigation the possible mechanism of thyrocytes disruption along with interruption of thyroid hormone biosynthesis by BS has been worked out. Commonly consumed BS, Bambusa Balcooa Roxb (BBR) water extract was analysed by GC MS; three doses below IC₅₀ were administered to thyrocytes in culture with and without iodine. Expressions of thyroglobulin (Tg), pendrin (PDS) and monocarboxylate transporter 8 (MCT8) were evaluated in thyrocytes with cell cycle analysis, reactive oxygen species (ROS) generation, DNA oxidation and apoptotic regulation through Bax, Bcl-2 and p53. Phytochemical analysis of BBR extract revealed the presence of precursors and metabolic end products of cyanogenic glycosides. Dose dependent decrease in expression of Tg and PDS with concomitant decrease in gene expression of these with MCT8 were observed. Increased ROS, DNA oxidation and associated imbalance were found through increased Bax and p53 with decreased Bcl-2 that perturbed thyrocytes cell cycle. Cyanogenic constituents of BBR generates ROS associated oxidative changes in thyrocytes with DNA damage and oxidation and cell cycle disruption followed by inhibition of thyroid hormone synthesizing regulatory elements; addition of extra iodine showed partial prevention.

即使在许多地区的盐加碘后，地方性甲状腺肿和相关的碘缺乏病（IDD）仍然是公共卫生的一个主要问题。其中，食用含氰植物是导致此类疾病持续存在的原因之一。正如我们早期的研究中已经报道的那样，竹笋（BS）就是一种含氰植物食品，它会导致某些甲状腺激素合成调节元件的破坏。在这项研究中，已经阐明了 BS 破坏甲状腺细胞以及干扰甲状腺激素生物合成的可能机制。采用 GC MS 分析常用的 BS、 Bambusa Balcooa Roxb (BBR) 水提取物；将低于IC _{50 的}三个剂量施用至含碘或不含碘的培养物中的甲状腺细胞。通过细胞周期分析、活性氧 (ROS) 生成、DNA 氧化以及通过 Bax、Bcl-2 和 p53 的细胞凋亡调节来评估甲状腺细胞中甲状腺球蛋白 (Tg)、pendrin (PDS) 和单羧酸转运蛋白 8 (MCT8) 的表达。 BBR 提取物的植物化学分析揭示了氰苷前体和代谢终产物的存在。观察到 Tg 和 PDS 表达随 MCT8 的剂量依赖性降低以及这些基因表达的伴随降低。通过 Bax 和 p53 的增加以及 Bcl-2 的减少发现了 ROS、DNA 氧化和相关失衡的增加，扰乱了甲状腺细胞的细胞周期。 BBR 的氰基成分会在甲状腺细胞中产生与 DNA 损伤、氧化和细胞周期破坏相关的氧化变化相关的 ROS，随后抑制甲状腺激素合成调节元件；添加额外的碘可以部分预防。