3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation.,Nano Letters

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3D Free-Standing Ordered Graphene Network Geometrically Regulates Neuronal Growth and Network Formation.
Nano Letters ( IF 9.6 ) Pub Date : 2020-09-11 , DOI: 10.1021/acs.nanolett.0c02107
Miao Xiao _{1,

2,

3} , Francesco Paolo Ulloa Severino ₄ , Federico Iseppon ₅ , Guosheng Cheng ₃ , Vincent Torre _{2,

6} , Mingliang Tang ₁

Affiliation

The control of cell–microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with a precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, they form well-defined 3D connections. Astrocytes have a more ramified shape similar to that seen in vivo because of the nanosized ripples and wrinkles on the surface of graphene skeleton. Neurons have axons and dendrites aligned along the graphene skeleton, allowing the formation of neuronal networks with highly controlled connections. Neuronal networks have higher electrical activity with functional signaling over a long distance along the graphene skeleton. Our study, for the first time, investigated the geometrical cues on ordered neuronal growth and network formation with the support of graphene in 3D, which therefore advanced the development of customized scaffolds for brain–machine interfaces or neuroprosthetic devices.

中文翻译：

3D自由有序石墨烯网络在几何上调节神经元的生长和网络的形成。

细胞-微环境相互作用的控制在构建用于组织工程的特定支架中起着关键作用。在这里，我们制作了具有精确定义的图案的3D独立式有序石墨烯（3D-OG）网络。在3D-OG支架上培养原代皮层细胞时，它们会形成定义明确的3D连接。星形胶质细胞具有类似于体内观察到的更分支的形状因为石墨烯骨架表面有纳米级的波纹和皱纹。神经元具有沿着石墨烯骨架排列的轴突和树突，从而形成具有高度受控连接的神经元网络。神经元网络在沿着石墨烯骨架的长距离上具有较高的电活动功能信号。我们的研究首次在3D石墨烯的支持下研究了有序神经元生长和网络形成的几何线索，因此推动了脑机接口或神经修复设备定制支架的开发。

更新日期：2020-10-15

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