Macrophages can associate with extracellular matrix (ECM) demonstrating nanosequenced cell-adhesive RGD ligand. In this study, we devised barcoded materials composed of RGD-coated gold and RGD-absent iron nanopatches to show various frequencies and position of RGD-coated nanopatches with similar areas of iron and RGD-gold nanopatches that maintain macroscale and nanoscale RGD density invariant. Iron patches were used for substrate coupling. Both large (low frequency) and externally positioned RGD-coated nanopatches stimulated robust attachment in macrophages, compared with small (high frequency) and internally positioned RGD-coated nanopatches, respectively, which mediate their regenerative/anti-inflammatory M2 polarization. The nanobarcodes exhibited stability in vivo. We shed light into designing ligand-engineered nanostructures in an external position to facilitate host cell attachment, thereby eliciting regenerative host responses.

中文翻译：

---

大的和外部定位的配体涂层纳米补丁促进了宿主巨噬细胞的粘附依赖性再生极化。

巨噬细胞可与细胞外基质（ECM）结合，显示出纳米序列的细胞粘附RGD配体。在这项研究中，我们设计了由带RGD涂层的金和不含RGD的铁纳米斑组成的条形码材料，以显示具有相似面积的铁和RGD-金纳米斑的RGD涂层纳米斑的各种频率和位置，从而保持宏观和纳米级RGD密度不变。铁贴片用于衬底耦合。与分别介导其再生/消炎M2极化的小（高频）和内部RGD涂层纳米贴片相比，大（低频）和外部RGD涂层纳米贴片均能刺激巨噬细胞牢固附着。纳米条形码在体内表现出稳定性。我们阐明了在外部位置设计配体工程化的纳米结构以促进宿主细胞附着，从而引发再生宿主反应的方法。