Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics.,ACS Nano

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Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics.
ACS Nano ( IF 15.8 ) Pub Date : 2020-09-11 , DOI: 10.1021/acsnano.0c04281
Tilman Schlotter ₁ , Sean Weaver ₁ , Csaba Forró _{1,

2} , Dmitry Momotenko ₁ , János Vörös ₁ , Tomaso Zambelli ₁ , Morteza Aramesh _{1,

3}

Affiliation

Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.

中文翻译：

用于单生物分子传感和单细胞分泌组学的动态纳米孔的受力控制形成。

对单核苷酸进行纳米孔传感已成为一种有前途的单分子技术，用于DNA测序和蛋白质组学。尽管纳米孔在概念上很简单，但是由于缺乏孔径可调性以及无法在复杂溶液中进行长期记录，这种技术在实际应用中的应用受到了限制。在这里，我们介绍了一种方法，该方法可以通过力控制调整在微流体装置（由氮化硅制成）的开口与软聚合物之间形成的纳米空间，来快速精确地按需形成尺寸可控制在2至20 nm之间的纳米孔基质。引入的纳米孔系统可在任意位置进行稳定的测量。

更新日期：2020-10-28

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