The main goal of this work was to evaluate the in vitro biological activity of two ferrocenyl chalcones (FcC-1 and FcC-2) against Haemonchus contortus (third-stage larvae (L3)) and Nacobbus aberrans (second-stage juveniles (J2)). Both compounds were synthesized and characterized by usual spectroscopic methods and their molecular structures were confirmed by single-crystal X-ray diffractometry. Nematode strains were examined in terms of percentage mortality of H. contortus (L3) by the action of FcC-1, which showed an effectivity of 100% at a concentration of 342 μM in 24 h, with EC50 = 20.33 μM and EC90 = 162.76 μM, whereas FcC-2 had an effectivity of 72% at a concentration of 342 μM in 24 h, with EC50 = 167.39 μM and EC90 = 316.21 μM. The effect of FcC-1 against nematode phytoparasite N. aberrans showed a better percentage of 95% at a concentration of 342 μM, with EC50 = 7.18 μM and EC90 = 79.25 μM, whereas the effect of FcC-2 was 87% at 342 μM, with EC50 = 168 μM and EC90 = 319.56 μM at 36 h. After treatment, the scanning electron micrographs revealed deformities in the dorsal flank and posterior part close to the tail of H. contortus L3. They showed moderate in vitro nematicidal activity against H. contortus L3 and N. aberrans J2.

中文翻译：

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两种二茂铁查尔酮对扭曲血矛线虫 (L3) 和 Nacobbus aberrans (J2) 幼虫的体外杀线虫活性

这项工作的主要目的是评估体外两种二茂铁查尔酮的生物活性（FcC-1和FcC-2） 反对扭曲血矛线虫（第三阶段幼虫（L3）） 和蝾螈（第二阶段少年（J2））。两种化合物均通过常规光谱方法合成和表征，其分子结构通过单晶 X 射线衍射法确认。线虫菌株根据死亡率百分比进行了检查H. contortus（大号3) 的行动FcC-1，在 342 μM 浓度下 24 小时内显示出 100% 的有效性，与 EC50= 20.33 μM 和 EC90= 162.76 μM，而FcC-2在 342 μM 浓度下 24 小时有效率为 72%，与 EC50= 167.39 μM 和 EC90= 316.21 微米。的效果FcC-1抗线虫植物寄生虫N. aberrans在 342 μM 的浓度下显示出更好的 95% 百分比，与 EC50= 7.18 μM 和 EC90= 79.25 μM，而FcC-2在 342 μM 时为 87%，EC50= 168 μM 和 EC90= 36 小时时为 319.56 μM。治疗后，扫描电子显微照片显示背侧和靠近尾部的后部畸形。H. contortus大号3. 他们表现出中等体外杀线虫活性H. contortus大号3和N. aberransĴ2.