Abstract

Aim

To study molecular diversity and genomic heritage of the Meitei community of Manipur using 20 autosomal gene loci markers.

Subjects and methods

Blood samples were collected from 120 unrelated, healthy individuals of the Meitei population following ethical standards. DNA was extracted using the Phenol chloroform organic extraction method and amplified using the PowerPlex^® 21 system. Genetic profiles of the individuals were generated using the Genetic Analyser 3500XL following the recommended protocol.

Results

The studied population showed Observed Heterozygosity (H_obs) from 0.583 (TH01) to 0.90 (D6S1043) among all the studied loci. The discrimination power and exclusion power for all the studied loci were found to be 1 and 0.9999999988, respectively, with the maximum power of discrimination being found at Penta E locus.

Conclusions

All the studied loci showed a high degree of matching probability and paternity index of 2.83 × 10⁻²⁴ and 7.35 × 10⁸, respectively; these are high-level statistical values and indicate that these loci might play a very important role in the application of DNA reports in the courts of justice. The studied population showed a relatively closer genetic affinity with Newar, Kathmandu, and Han Chinese populations compared with the South and West Indian populations. The outcomes of this study will enrich the STR database of the Indian population and this is the first global report on genetic diversity in the Meitei community of Manipur, India, at 20 autosomal STR genetic markers.

中文翻译：

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使用常染色体STRs评估Manipur（印度）Meitei社区与东亚人群的基因组多样性和共同祖先。

摘要

目标

使用20个常染色体基因位点标记来研究曼尼普尔邦Meitei社区的分子多样性和基因组遗传。

主题与方法

遵循道德标准从梅特伊（Meitei）人口的120名无亲缘关系的健康个体中采集血液样本。使用酚氯仿有机提取方法提取DNA并使用的PowerPlex扩增^® 21系统。按照推荐的方案，使用遗传分析仪3500XL生成个体的遗传概况。

结果

在所有研究的基因座中，观察到的杂合度（H _obs）从0.583（TH01）至0.90（D6S1043）。发现所有研究基因座的鉴别力和排除力分别为1和0.9999999988，在Penta E基因座处发现最大的鉴别力。

结论

所有研究的基因座均显示出较高的匹配概率和亲子鉴定指数，分别为2.83×10 ^-24和7.35×10 ⁸。这些是高水平的统计值，表明这些基因座可能在法院的DNA报告应用中起非常重要的作用。与南印度和西印度人口相比，研究的人口与纽瓦，加德满都和汉族人口的遗传亲和力相对较近。这项研究的结果将丰富印度人口的STR数据库，这是关于印度Manipur Meitei社区20个常染色体STR遗传标记的遗传多样性的第一份全球报告。