ABSTRACT

Introduction

Viruses induce profound changes in the cells they infect. Understanding these perturbations will assist in designing better therapeutics to combat viral infection. System-based proteomic assays now provide unprecedented opportunity to monitor large numbers of cellular proteins.

Areas covered

This review will describe various quantitative and functional mass spectrometry-based methods, and complementary non-mass spectrometry-based methods, such as aptamer profiling and proximity extension assays, and examples of how each are used to delineate how viruses affect host cells, identify which viral proteins interact with which cellular proteins, and how these change during the course of a viral infection. PubMed was searched multiple times prior to manuscript submissions and revisions, using virus, viral, proteomics; in combination with each keyword. The most recent examples of published works from each search were then analyzed.

Expert opinion

There has been exponential growth in numbers and types of proteomic analyses in recent years. Continued development of reagents that allow increased multiplexing and deeper proteomic probing of the cell, at quantitative and functional levels, enhancements that target more important protein modifications, and improved bioinformatics software tools and pathway prediction algorithms will accelerate this growth and usher in a new era of host proteome understanding.

中文翻译：

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蛋白质组学方法的更新，以揭示病毒感染过程中病毒诱导的蛋白质改变和病毒-宿主蛋白质相互作用。

摘要

介绍

病毒会导致它们感染的细胞发生深刻的变化。了解这些扰动将有助于设计更好的疗法来对抗病毒感染。现在，基于系统的蛋白质组学分析为监测大量细胞蛋白质提供了前所未有的机会。

覆盖区域

本综述将描述各种基于定量和功能质谱的方法，以及基于非质谱的补充方法，例如适体分析和邻近延伸分析，以及如何使用每种方法描述病毒如何影响宿主细胞的示例，确定哪些病毒蛋白与哪些细胞蛋白相互作用，以及这些蛋白在病毒感染过程中如何变化。PubMed 在手稿提交和修改之前被多次搜索，使用病毒、病毒、蛋白质组学；结合每个关键字。然后分析来自每次搜索的已发表作品的最新示例。

专家意见

近年来，蛋白质组学分析的数量和类型呈指数增长。在定量和功能水平上允许增加多路复用和更深入的细胞蛋白质组学探测的试剂的持续开发、针对更重要蛋白质修饰的增强以及改进的生物信息学软件工具和通路预测算法将加速这种增长并迎来一个新时代宿主蛋白质组理解。