Six species of the genus Bursaphelenchus were recovered as the result of a survey to assess the potential introduction of Bursaphelenchus xylophilus into the conifer forests of Romania. Bursaphelenchus xylophilus was not detected in the sampling regions. Bursaphelenchus dietrichi was recovered from one point, representing the first report of this species in the country. The other detected species, B. abietinus, B. andrassyi, B. hofmanni, B. fraudulentus and B. willibaldi, were characterized by their morphometric data and molecular analyses. The morphometrics of Romanian populations of B. hofmanni and B. willibaldi are presented for the first time. The phylogenetic relationships of the recovered species (except for B. abietinus, with no successful sequencing) were reconstructed using the sequences of the D2‐D3 expansion segments of the large subunit of ribosomal DNA (D2‐D3LSU rDNA). Some of the species were also characterized using the restriction fragment length polymorphism (RFLP) pattern of the internal transcribed spacer (ITS) fragment. The distribution, related host trees and phylogenetic relationships of the identified species are discussed.

中文翻译：

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关于Bursaphelenchus spp发生和分布的新观察。（Nematoda：Aphelenchoididae）在罗马尼亚的针叶树中

一项调查的结果是，共有六种Bursaphelenchus属被发现，以评估可能将Xylophilus Bursaphelenchus引入罗马尼亚的针叶林。在采样区域未检测到嗜木伯氏菌。从一个点恢复到了Bursaphelenchus Dietrichi，这是该物种在该国的首次报道。其它检测到的种类，乙。 Abietinus，B。 安德拉西，B。 霍夫曼尼，B。 中伪和乙。 威利巴第，通过形态学数据和分子分析来表征。罗马尼亚B族的形态计量学。 hofmanni和乙。 willibaldi第一次出现。将回收的物质的系统发育关系（除了乙。 abietinus，没有成功测序）用大亚基核糖体DNA（D2-D3LSU基因）的D2-D3扩张段的序列重构。还使用内部转录间隔区（ITS）片段的限制性片段长度多态性（RFLP）模式对某些物种进行了表征。讨论了已鉴定物种的分布，相关寄主树和系统发育关系。