Toll-like receptor 2 mediates the immune response of the bovine oviductal ampulla to sperm binding.,Molecular Reproduction and Development

当前位置： X-MOL 学术 › Mol. Reprod. Dev. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Toll-like receptor 2 mediates the immune response of the bovine oviductal ampulla to sperm binding.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-09-10 , DOI: 10.1002/mrd.23422
Vernadyn A Morillo _{1,

2} , Ihshan Akthar ₁ , Mariani F Fiorenza _{1,

3} , Ken-Ichi Takahashi ₄ , Motoki Sasaki ₅ , Mohamed A Marey _{1,

6} , Susan S Suarez ₇ , Akio Miyamoto ₁

Affiliation

We previously reported that sperm binding to cultured bovine oviduct epithelial cells induces an anti‐inflammatory immune response. Now we have developed a differentiated explant model to focus on the oviductal ampulla, where fertilization occurs, and to study the effect of sperm capacitation on the immune response. We used heparin to stimulate bovine sperm capacitation. Fluorescence imaging showed that 5,5ʹ,6,6ʹ‐tetrachloro‐1,1ʹ,3,3ʹ‐tetraethylbenzimidazolyl‐carbocyanine iodide‐labeled sperm pretreated with (Hep⁽⁺⁾) or without (Hep⁽⁻⁾) heparin rapidly attached to the explant ciliated epithelium in similar numbers. However, only Hep⁽⁺⁾ sperm upregulated explant messenger RNA (mRNA) transcription of TLR2, IL8, TGFB1, and PGES, without changes in TNFA and IL‐10 expression, while Hep⁽⁻⁾ sperm only upregulated PGES. The responses were primarily anti‐inflammatory, with a greater response produced by Hep⁽⁺⁾ sperm, which also produced a substantial increase in TLR2 protein expression in the epithelium. The addition of TLR1/2 (toll‐like receptor 1/2) antagonist to the Hep⁽⁺⁾ and (Hep⁽⁻⁾) sperm‐explant coincubations reduced sperm attachment to the epithelium and inhibited TLR2 protein expression and some of the Hep⁽⁺⁾ sperm‐induced mRNA transcription. Our observations suggest that the ampullar epithelium immunologically reacts more strongly to sperm that have undergone heparin stimulation of capacitation. This anti‐inflammatory response could serve to protect capacitated sperm as they approach the oocyte in the ampulla.

中文翻译：

Toll 样受体 2 介导牛输卵管壶腹对精子结合的免疫反应。

我们之前报道过精子与培养的牛输卵管上皮细胞结合诱导抗炎免疫反应。现在我们已经开发了一个分化的外植体模型，专注于发生受精的输卵管壶腹，并研究精子获能对免疫反应的影响。我们使用肝素来刺激牛精子获能。荧光成像显示，用 (Hep ⁽⁺⁾ ) 或不用 (Hep ⁽^-) ) 肝素预处理的 5,5ʹ,6,6ʹ-四氯-1,1ʹ,3,3ʹ-四乙基苯并咪唑基-碳菁碘化物标记的精子迅速附着在以相似的数量外植纤毛上皮。然而，只有 Hep ⁽⁺⁾精子上调TLR2、IL8、TGFB1和PGES，在不改变TNFA和IL-10表达，而的Hep ^（^{- ）}精子只上调PGES。这些反应主要是抗炎的，Hep ⁽⁺⁾精子产生了更大的反应，这也使上皮细胞中的 TLR2 蛋白表达显着增加。将 TLR1/2（toll 样受体 1/2）拮抗剂添加到 Hep ^（+）和（Hep ^（^-））精子-外植体共孵化减少了精子对上皮的附着并抑制了 TLR2 蛋白表达和一些 Hep ^{（ +)}精子诱导的 mRNA 转录。我们的观察表明壶腹上皮对已经历肝素刺激获能的精子的免疫反应更强烈。当精子接近壶腹中的卵母细胞时，这种抗炎反应可以保护精子。

更新日期：2020-10-30

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11