Root canal filling aims at eliminating empty spaces into the root canal system using biologically compatible materials. Three‐dimensional root canal obturation must prevent or minimize the reinfection caused by microorganisms' leakage. This study aimed at evaluating whether fluorophore (Rhodamine or Fluo‐3) influences the CLSM images of intratubular penetration of four endodontic sealers. Eighty bovine teeth were prepared using K files up to a size #70 and irrigated with 2.5% sodium hypochlorite. All roots were divided into eight groups (n = 10) according to the sealer and fluorophore used: AH Plus/Rhodamine, AH Plus/Fluo‐3, Sealer Plus/Rhodamine, Sealer Plus/Fluo‐3, Sealer Plus BC/Rhodamine, Sealer Plus BC/Fluo‐3, Endosequence/Rhodamine, and Endosequence/Fluo‐3. All roots were filled using cold lateral compaction technique. After 7 days, the roots were transversely sectioned, and three slices, one of each canal third, were obtained. Intratubular penetration was evaluated using CLSM. Sealer Plus BC/Rhodamine and Endosequence BC/Rhodamine presented higher intratubular penetration than AH Plus/Fluo‐3 and Sealer Plus/Fluo‐3 (p ˂ .05). The intragroup analysis showed similar intratubular penetration, regardless of the root third, except for the apical third in AH Plus/Fluo‐3 and Sealer Plus BC/Fluo‐3 groups. The type of fluorophore influences the calcium silicate sealers' tubular penetration but not of epoxy resin‐based ones using CLSM. Bioceramic sealers should not be used associated with Rhodamine for CLSM evaluation.

中文翻译：

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牙髓封闭剂的管内渗透取决于用于 CLSM 评估的荧光团。

根管充填旨在使用生物相容性材料消除根管系统中的空隙。三维根管封闭必须防止或最大限度地减少微生物渗漏引起的再感染。本研究旨在评估荧光团（罗丹明或 Fluo-3）是否影响四种牙髓封闭剂的管内穿透的 CLSM 图像。使用 K 锉制备 80 颗牛牙，最大尺寸为 #70，并用 2.5% 次氯酸钠冲洗。所有的根被分成八组（n= 10) 根据使用的封闭剂和荧光团：AH Plus/Rhodamine、AH Plus/Fluo-3、Sealer Plus/Rhodamine、Sealer Plus/Fluo-3、Sealer Plus BC/Rhodamine、Sealer Plus BC/Fluo-3、Endosequence /罗丹明和 Endosequence/Fluo-3。使用冷横向压实技术填充所有根。7 天后，横切根部，获得三片，每根管三分之一。使用 CLSM 评估管内穿透。封口机加BC /若丹明和Endosequence BC /罗丹明呈现比AH加/的Fluo-3和封口机加/的Fluo-3（管内的高穿透p˂ .05)。除了 AH Plus/Fluo-3 和 Sealer Plus BC/Fluo-3 组中的根尖 1/3 外，组内分析显示类似的管内渗透率，无论根 1/3 是多少。荧光团的类型影响硅酸钙封闭剂的管状渗透，但不影响使用 CLSM 的环氧树脂基封闭剂。生物陶瓷密封剂不应与罗丹明一起用于 CLSM 评估。