Effect of Photocaged Isopropyl β‐d‐1‐thiogalactopyranoside Solubility on the Light Responsiveness of LacI‐controlled Expression Systems in Different Bacteria,ChemBioChem

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Effect of Photocaged Isopropyl β‐d‐1‐thiogalactopyranoside Solubility on the Light Responsiveness of LacI‐controlled Expression Systems in Different Bacteria
ChemBioChem ( IF 2.6 ) Pub Date : 2020-09-11 , DOI: 10.1002/cbic.202000377
Fabian Hogenkamp ₁ , Fabienne Hilgers ₂ , Andreas Knapp ₂ , Oliver Klaus ₂ , Claus Bier ₁ , Dennis Binder ₂ , Karl-Erich Jaeger _{2,

3} , Thomas Drepper ₂ , Jörg Pietruszka _{1,

3}

Affiliation

Extended toolbox: New photocaged IPTG derivatives with various hydrophobicities for light‐induced gene expression were synthesised and tested with three different expression host strains. The most water‐soluble compound showed superior performance, especially in Bacillus subtilis.