Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and their interactions with human lectins using NMR spectroscopy.,Angewandte Chemie International Edition

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Structural characterization of the N-linked glycans in the receptor binding domain of the SARS-CoV-2 spike protein and their interactions with human lectins using NMR spectroscopy.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-09-11 , DOI: 10.1002/anie.202011015
Maria Pia Lenza ₁ , Iker Oyenarte ₁ , Tammo Diercks ₁ , Jon Imanol Quintana ₁ , Ana Gimeno ₁ , Helena Coelho ₂ , Ana Diniz ₂ , Francesca Peccati ₁ , Sandra Delgado ₁ , Alexandre Bosch ₁ , Mikel Valle ₁ , Oscar Millet ₁ , Nicola G A Abrescia _{1,

3,

4} , Asís Palazón _{1,

3} , Filipa Marcelo ₂ , Gonzalo Jiménez-Osés ₁ , Jesús Jiménez-Barbero _{1,

3,

5} , Ana Ardá ₁ , June Ereño-Orbea _{1,

3}

Affiliation

The glycan structures of the receptor binding domain of the SARS‐CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR. The different possible interacting epitopes have been deeply analysed and characterized, providing evidence of the presence of glycan structures not found in previous MS‐based analyses. The interaction of the RBD ¹³C‐labelled glycans with different human lectins, which are expressed in different organs and tissues that may be affected during the infection process, has also been evaluated by NMR. In particular, ¹⁵N‐labelled galectins (galectins‐3, ‐7 and ‐8 N‐terminal), Siglecs (Siglec‐8, Siglec‐10), and C‐type lectins (DC‐SIGN, MGL) have been employed. Complementary experiments from the glycoprotein perspective or from the lectin's point of view have permitted to disentangle the specific interacting epitopes in each case. Based on these findings, 3D models of the interacting complexes have been proposed.

中文翻译：

使用 NMR 光谱法对 SARS-CoV-2 刺突蛋白受体结合域中的 N 连接聚糖进行结构表征，及其与人类凝集素的相互作用。

使用 NMR 研究了人 HEK293F 细胞中表达的 SARS-CoV2 刺突糖蛋白的受体结合域的聚糖结构。不同的可能相互作用的表位已被深入分析和表征，提供了以前基于质谱的分析中未发现的聚糖结构存在的证据。 RBD ¹³ C 标记聚糖与不同人类凝集素的相互作用也已通过 NMR 进行了评估，这些凝集素在感染过程中可能受到影响的不同器官和组织中表达。特别是，使用了^15种N标记的半乳糖凝集素（半乳糖凝集素-3、-7和-8 N端）、Siglecs（Siglec-8、Siglec-10）和C型凝集素（DC-SIGN、MGL）。从糖蛋白的角度或从凝集素的角度进行的补充实验可以解开每种情况下特定的相互作用表位。基于这些发现，提出了相互作用复合物的 3D 模型。

更新日期：2020-09-11

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