Abstract In this study, eco-friendly and green synthesis of noble-metal nanoparticles using C. monogyna leaf extract (CML@X-NPs, X= Ag, Au) as capping agent and reducing agent were reported. Various conditions including pH, temperature, concentration and contact time to enhance biological assay efficiency and mean size of CML@X-NPs were studied. The as-synthesized products were characterized by TEM, FE-SEM, XRD, EDS, FT-IR and UV–Vis techniques. The uniform, spherical shape and homogeneous of CML@X-NPs with a grain size of 40–60 nm was observed from TEM and FE-SEM images. In order to determined antibacterial and antibiotic resistance activities, ATCC and multidrug-resistant isolates were identified and evaluated by micro-broth dilution method. Due to the results, the MIC values for synthesized CML@Ag-NPs and CML@Au-NPs against S. aureus, E. faecalis, P. aeruginosa, A. baumannii, E. coli, K. pneumonia and P. mirabilis were 0.29 and 750 µg/ml, 0.5 and 185 µg/ml, 0.29 and 750 µg/ml, 0.29 and 1500 µg/ml, 0.29 and 750 µg/ml, 0.5 and 370 µg/ml, 0.2 and 750 µg/ml, respectively. The cytotoxicity of the synthesized CML@Ag-NPs and CML@Au-NPs were performed using MTT assay against gastric (AGS) and breast (MCF-7) cancer cell lines. The anticancer results revealed that CML@X-NPs had significant anticancer effects against human cancer cell lines. The facile, none-toxic, fast and green synthesis of noble metal nanoparticles in presence of natural reducing agent raises their potential for biological activities like anticancer and antibacterial.

中文翻译：

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使用山楂叶提取物（CML@X-NPs，X=Ag，Au）生物合成贵金属纳米颗粒：对乳腺癌和胃癌细胞系的抗菌和细胞毒活性

摘要 在本研究中，报道了以单葛叶提取物（CML@X-NPs，X=Ag，Au）为封端剂和还原剂的贵金属纳米颗粒的环保绿色合成。研究了各种条件，包括 pH、温度、浓度和接触时间，以提高 CML@X-NPs 的生物测定效率和平均大小。合成的产物通过 TEM、FE-SEM、XRD、EDS、FT-IR 和 UV-Vis 技术进行表征。从 TEM 和 FE-SEM 图像中观察到晶粒尺寸为 40-60 nm 的均匀、球形和均质的 CML@X-NP。为了确定抗菌和抗生素抗性活性，通过微量肉汤稀释法鉴定和评估ATCC和多重耐药分离株。由于结果，合成的 CML@Ag-NPs 和 CML@Au-NPs 对金黄色葡萄球菌、大肠杆菌的 MIC 值。粪菌、铜绿假单胞菌、鲍曼不动杆菌、大肠杆菌、肺炎克雷伯菌和奇异假单胞菌分别为 0.29 和 750 微克/毫升、0.5 和 185 微克/毫升、0.29 和 750 微克/毫升、0.29 和 1500 微克/毫升，分别为 0.29 和 750 µg/ml、0.5 和 370 µg/ml、0.2 和 750 µg/ml。使用 MTT 法测定合成的 CML@Ag-NPs 和 CML@Au-NPs 对胃癌（AGS）和乳腺癌（MCF-7）癌细胞系的细胞毒性。抗癌结果表明，CML@X-NPs对人类癌细胞系具有显着的抗癌作用。在天然还原剂的存在下，贵金属纳米颗粒的简便、无毒、快速和绿色合成提高了其抗癌和抗菌等生物活性的潜力。分别为 0.29 和 750 µg/ml、0.5 和 370 µg/ml、0.2 和 750 µg/ml。使用 MTT 法测定合成的 CML@Ag-NPs 和 CML@Au-NPs 对胃癌（AGS）和乳腺癌（MCF-7）癌细胞系的细胞毒性。抗癌结果表明，CML@X-NPs对人类癌细胞系具有显着的抗癌作用。在天然还原剂的存在下，贵金属纳米颗粒的简便、无毒、快速和绿色合成提高了其抗癌和抗菌等生物活性的潜力。分别为 0.29 和 750 µg/ml、0.5 和 370 µg/ml、0.2 和 750 µg/ml。使用 MTT 法测定合成的 CML@Ag-NPs 和 CML@Au-NPs 对胃癌（AGS）和乳腺癌（MCF-7）癌细胞系的细胞毒性。抗癌结果表明，CML@X-NPs对人类癌细胞系具有显着的抗癌作用。在天然还原剂的存在下，贵金属纳米颗粒的简便、无毒、快速和绿色合成提高了其抗癌和抗菌等生物活性的潜力。抗癌结果表明，CML@X-NPs对人类癌细胞系具有显着的抗癌作用。在天然还原剂的存在下，贵金属纳米颗粒的简便、无毒、快速和绿色合成提高了其抗癌和抗菌等生物活性的潜力。抗癌结果表明，CML@X-NPs对人类癌细胞系具有显着的抗癌作用。在天然还原剂的存在下，贵金属纳米颗粒的简便、无毒、快速和绿色合成提高了其抗癌和抗菌等生物活性的潜力。