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Carboxymethyl cellulose supported green synthetic features of gold nanoparticles: Antioxidant, cell viability, and antibacterial effectiveness
Synthetic Metals ( IF 4.0 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.synthmet.2020.116553
Moustafa M.G. Fouda , Jamaan S Ajarem , Saleh N Maodaa , Ahmed A Allam , Mohamed M. Taher , M.K. Ahmed

Abstract Gold nanoparticles (AuNPs) are widely used in the diagnosis and treatment of diseases owing to their environmental friendliness and controllability due to their compatibility and high potency in drug delivery. Here, AuNPs were prepared from carboxymethyl cellulose (CMC) and showed an extensive absorption peak at 528 nm owing to the characterized Plasmon Surface Resonance. TEM measurement affirms that the Au particles had spherical shapes accompanied by a uniform distribution and a size of approximately 12-20 nm. Otherwise, SEM images showed that the AuNPs were configured in agglomerated spherical shapes and the crystalline phase was evident from SAED images and spikes in the XRD model. Vigorous antioxidant was tested using the DPPH scavenger analysis and the produced maximum trapping capacity (241.1 ± 13.22 mg/g) was reached at an AuNPs content of 106.25 mg/mL. The ratio of viable cells for AuNPs by HEK 293 T cell lines was reduced to 75.1 ± 3.8% at an approximated concentration of 500 μg / mL. Besides, the antibacterial potency was examined after one day of exposure to (E. coli and P. aeruginosa) and (S. aureus and B. subtilis). AuNPs were toxic against all selected bacteria. P. aeruginosa was the most susceptible bacterium to AuNPs with maximum inhibition zones of 50% whereas B. subtilis at 2 mg/L concentration of AuNPs takes the lowest percentages of inhibition zone with 30% compared to 25% for antibiotic standard. These extraordinary behaviors of AuNPs exhibits the manipulation of nanoparticles is highly recommended for universal use in the treatment of skin and wounds.

中文翻译:

羧甲基纤维素支持金纳米粒子的绿色合成特性:抗氧化、细胞活力和抗菌效果

摘要 金纳米粒子(AuNPs)由于其相容性和高效的药物递送,具有环境友好性和可控性,被广泛用于疾病的诊断和治疗。在这里,AuNPs 由羧甲基纤维素 (CMC) 制备,并且由于表征的等离子体表面共振在 528 nm 处显示出广泛的吸收峰。TEM 测量证实 Au 颗粒具有球形形状, 伴随着均匀分布和大约 12-20 nm 的尺寸。否则,SEM 图像显示 AuNP 被配置为团聚的球形,并且从 SAED 图像和 XRD 模型中的尖峰可以明显看出结晶相。使用 DPPH 清除剂分析和产生的最大捕集能力 (241.1 ± 13. 22 mg/g) 在 AuNPs 含量为 106.25 mg/mL 时达到。在大约 500 μg/mL 的浓度下,HEK 293 T 细胞系对 AuNP 的活细胞比率降低至 75.1 ± 3.8%。此外,在暴露于(大肠杆菌和铜绿假单胞菌)和(金黄色葡萄球菌和枯草芽孢杆菌)一天后检查抗菌效力。AuNPs 对所有选定的细菌都有毒性。铜绿假单胞菌是对 AuNPs 最敏感的细菌,最大抑制区为 50%,而 2mg/L AuNPs 浓度的枯草芽孢杆菌的抑制区百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。在大约 500 μg/mL 的浓度下,HEK 293 T 细胞系对 AuNP 的活细胞比率降低至 75.1 ± 3.8%。此外,在暴露于(大肠杆菌和铜绿假单胞菌)和(金黄色葡萄球菌和枯草芽孢杆菌)一天后检查抗菌效力。AuNPs 对所有选定的细菌都有毒性。铜绿假单胞菌是对 AuNPs 最敏感的细菌,最大抑制区为 50%,而 2mg/L AuNPs 浓度的枯草芽孢杆菌的抑制区百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。在大约 500 μg/mL 的浓度下,HEK 293 T 细胞系对 AuNP 的活细胞比率降低至 75.1 ± 3.8%。此外,在暴露于(大肠杆菌和铜绿假单胞菌)和(金黄色葡萄球菌和枯草芽孢杆菌)一天后检查抗菌效力。AuNPs 对所有选定的细菌都有毒性。铜绿假单胞菌是对 AuNPs 最敏感的细菌,最大抑制区为 50%,而 2mg/L AuNPs 浓度的枯草芽孢杆菌的抑制区百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。在暴露于(大肠杆菌和铜绿假单胞菌)和(金黄色葡萄球菌和枯草芽孢杆菌)一天后检查抗菌效力。AuNPs 对所有选定的细菌都有毒性。铜绿假单胞菌是对 AuNPs 最敏感的细菌,最大抑制区为 50%,而 2mg/L AuNPs 浓度的枯草芽孢杆菌的抑制区百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于治疗皮肤和伤口。在暴露于(大肠杆菌和铜绿假单胞菌)和(金黄色葡萄球菌和枯草芽孢杆菌)一天后检查抗菌效力。AuNPs 对所有选定的细菌都有毒性。铜绿假单胞菌是对 AuNPs 最敏感的细菌,最大抑制区为 50%,而 2mg/L AuNPs 浓度的枯草芽孢杆菌的抑制区百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。2 mg/L AuNPs 浓度的 subtilis 抑制区的百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。2 mg/L AuNPs 浓度的 subtilis 抑制区的百分比最低,为 30%,而抗生素标准为 25%。AuNPs 的这些非凡行为表现出对纳米粒子的操纵,强烈建议普遍用于皮肤和伤口的治疗。
更新日期:2020-11-01
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