The potential capability to rapid, highly sensitive, and selective diagnose neurotransmitters in human serum environment is extremely crucial for clinical biology. Herein an electrochemical sensor based on Ag nanoparticles anchored onto CuO porous nanobelts (Ag/CuO PNBs) was constructed for the ultrasensitive determination of dopamine (DA), which was prepared via a facile two-step cation-exchange reaction followed by in-situ thermal conversion. The Ag/CuO PNBs exhibited an excellent electrochemical sensing performance to DA. The linear range for the detection of DA was ranged from 0.04 to 10 μM with an outstanding detection limit of 7.0 nM. Cyclic voltammograms (CVs) were employed to investigate kinetic information of multiphase electron transfer reactions, revealing that Ag nanoparticles significantly enhanced electrocatalytic activity toward DA owing to the participation of Ag(0)/Ag(I) cycle. Meanwhile, the Ag/CuO PNBs showed the significant selectivity, remarkable stability and repeatability, as well as attractive reproducibility (RSD ≤ 3.7 %). Furthermore, the practicality of the developed sensor has been evaluated by analyzing DA in serum-containing electrolyte, and the recovery in the range of 95.7∼111.1 % was obtained. The results confirmed that the as-prepared sensor presented a significant promise for the detection of DA in real sample analysis.

在人类血清环境中快速，高度敏感和选择性诊断神经递质的潜在能力对于临床生物学至关重要。本文中，构建了一种基于银纳米颗粒的电化学传感器，该传感器固定在CuO多孔纳米带（Ag / CuO PNB）上，用于多巴胺（DA）的超灵敏测定，该传感器是通过简便的两步阳离子交换反应然后原位热制备的转换。Ag / CuO PNB对DA表现出优异的电化学感应性能。DA检测的线性范围为0.04至10μM，检测限为7.0 nM。循环伏安图（CV）用于研究多相电子转移反应的动力学信息，揭示了由于Ag（0）/ Ag（I）循环的参与，Ag纳米粒子显着增强了对DA的电催化活性。同时，Ag / CuO PNB表现出显着的选择性，显着的稳定性和可重复性，以及有吸引力的重现性（RSD≤3.7％）。此外，已通过分析含血清电解质中的DA评估了开发的传感器的实用性，并获得了95.7〜111.1％的回收率。结果证实，所制备的传感器为真实样品分析中的DA检测提供了重大希望。通过分析含血清电解质中的DA评估了开发的传感器的实用性，并且回收率达到95.7〜111.1％。结果证实，所制备的传感器为真实样品分析中的DA检测提供了重大希望。通过分析含血清电解质中的DA评估了开发的传感器的实用性，并且回收率达到95.7〜111.1％。结果证实，所制备的传感器为真实样品分析中的DA检测提供了重大希望。